添加海藻糖、维生素C和维生素E的精液稀释剂对小型猪冻融后精子质量的影响
EFFECT OF SEMEN EXTENDER SUPPLEMENTATION WITH TREHALOSE，VITAMIN C AND E ON POST-THAW MIN PIG SPERM QUALITIES.
作者信息
Ma H, Liu D, Wang W, Wang L, Fu Bo, Li Z, He X
机构信息
Heilongjiang Academy of Agricultural Sciences, Harbin, China.
出版信息
Cryo Letters. 2015 Sep-Oct;36(5):308-12.
BACKGROUND
Cryopreservation of min pig sperm offers an effective method to protect this valuable genetic resource.
OBJECTIVE
To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig.
MATERIALS AND METHODS
Ejaculated min pig sperm frozen in lactose-egg yolk (LEY) extender supplemented with trehalose, vitamin C and/or water-soluble vitamin E were thawed, and the quality of sperm samples were evaluated.
RESULTS
The quality of post-thawed sperm samples was higher in the groups with supplementation than that of the control group (P < 0.05). Furthermore, the addition of 100 mM trehalose and 25 mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm (P < 0.05).
CONCLUSION
Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.
背景
小型猪精子的冷冻保存为保护这一宝贵的遗传资源提供了一种有效方法。
目的
确定在稀释液中添加某些抗氧化剂是否能够提高小型猪冻融后精子的质量。
材料与方法
将采集的小型猪精液冷冻于添加了海藻糖、维生素C和/或水溶性维生素E的乳糖-蛋黄（LEY）稀释液中，解冻后评估精子样本的质量。
结果
添加组冻融后精子样本的质量高于对照组（P < 0.05）。此外，在冷冻稀释液中添加100 mM海藻糖和25 mM水溶性维生素E能有效保持精子质量（P < 0.05）。
结论
冷冻稀释液中的海藻糖和水溶性维生素E能有效提高小型猪精子冻融后的质量。
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