Abdussamad A M, Gauly M, Holtz W
Current address: Department of Animal Science, Faculty of Agriculture, Bayero University, Kano, Kano State, Nigeria.
Faculty of Science and Technology, Free University of Bolzano, Bozen, Italy.
Cryo Letters. 2015 Jul-Aug;36(4):278-84.
Two experiments were conducted. The purpose of Experiment 1 was to investigate whether viability of bovine semen stored in liquid nitrogen (-196°C) will be adversely affected by temporary exposure to dry ice (-79°C). It was convincingly shown that post thaw-motility was not affected, regardless whether semen was thawed immediately or after being returned to liquid nitrogen. Shipping or temporary storage on dry ice, thus, is a viable option. In Experiment 2, refreezing of frozen-thawed semen was attempted. The proportion of motile spermatozoa was reduced by a factor of ten to between 6.0 % and 7.4 %, regardless whether thawing occurred directly after removal from liquid nitrogen or after an interim period on dry ice. When semen was refrozen on dry ice before being returned to liquid nitrogen, motility rates were significantly improved (13.0 % to 17.0 %, P<0.05). In both experiments sperm cells that remained motile displayed vigorous forward movement and normal morphological appearance.
进行了两项实验。实验1的目的是研究临时暴露于干冰（-79°C）是否会对储存在液氮（-196°C）中的牛精液活力产生不利影响。结果令人信服地表明，解冻后的活力不受影响，无论精液是立即解冻还是在返回液氮后解冻。因此，用干冰运输或临时储存是一种可行的选择。在实验2中，尝试对冻融后的精液进行再次冷冻。无论精液是从液氮中取出后直接解冻还是在干冰上经过一段时间后解冻，活动精子的比例都降低了十分之一，降至6.0%至7.4%之间。当精液在返回液氮之前在干冰上再次冷冻时，活力率显著提高（13.0%至17.0%，P<0.05）。在两项实验中，仍具活力的精子细胞均表现出有力的向前运动和正常的形态外观。
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