Division of Paediatric Respiratory Medicine, University Children's Hospital, Bern, Switzerland Dept of Clinical Research, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
Division of Paediatric Respiratory Medicine, University Children's Hospital, Bern, Switzerland Dept of Clinical Research, University of Bern, Bern, Switzerland.
Eur Respir J. 2016 Feb;47(2):520-30. doi: 10.1183/13993003.00665-2015. Epub 2015 Nov 19.
Vitamin D has immunomodulatory properties in the defence against pathogens. Its insufficiency is a widespread feature of cystic fibrosis (CF) patients, which are repeatedly suffering from rhinovirus (RV)-induced pulmonary exacerbations.To investigate whether vitamin D has antiviral activity, primary bronchial epithelial cells from CF children were pre-treated with vitamin D and infected with RV16. Antiviral and anti-inflammatory activity of vitamin D was assessed. RV and LL-37 levels were measured in bronchoalveolar lavage (BAL) of CF children infected with RV.Vitamin D reduced RV16 load in a dose-dependent manner in CF cells (10(-7 )M, p<0.01). The antiviral response mediated by interferons remained unchanged by vitamin D in CF cells. Vitamin D did not exert anti-inflammatory properties in RV-infected CF cells. Vitamin D increased the expression of the antimicrobial peptide LL-37 up to 17.4-fold (p<0.05). Addition of exogenous LL-37 decreased viral replication by 4.4-fold in CF cells (p<0.05). An inverse correlation between viral load and LL-37 levels in CF BAL (r=-0.48, p<0.05) was observed.RV replication in primary CF bronchial cells was reduced by vitamin D through the induction of LL-37. Clinical studies are needed to determine the importance of an adequate control of vitamin D for prevention of virus-induced pulmonary CF exacerbations.
维生素 D 具有抗感染的免疫调节特性。囊性纤维化（CF）患者普遍存在维生素 D 不足的情况，他们会反复因鼻病毒（RV）引起的肺部恶化。为了研究维生素 D 是否具有抗病毒活性，用维生素 D 预处理 CF 儿童的原代支气管上皮细胞，然后用 RV16 感染。评估了维生素 D 的抗病毒和抗炎活性。RV 和 LL-37 水平在感染 RV 的 CF 儿童的支气管肺泡灌洗液（BAL）中进行了测量。维生素 D 以剂量依赖性方式降低 CF 细胞中的 RV16 载量（10(-7 )M，p<0.01）。在 CF 细胞中，维生素 D 对干扰素介导的抗病毒反应没有影响。维生素 D 在感染 RV 的 CF 细胞中没有发挥抗炎作用。维生素 D 使抗菌肽 LL-37 的表达增加了 17.4 倍（p<0.05）。外源性 LL-37 的添加使 CF 细胞中的病毒复制减少了 4.4 倍（p<0.05）。在 CF BAL 中观察到病毒载量与 LL-37 水平之间呈负相关（r=-0.48，p<0.05）。维生素 D 通过诱导 LL-37 减少了原代 CF 支气管细胞中的 RV 复制。需要进行临床研究以确定适当控制维生素 D 对预防病毒引起的 CF 肺部恶化的重要性。
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