砷在污染土壤中的相对生物有效性：动物模型、给药方案和生物学终点的比较。
Arsenic Relative Bioavailability in Contaminated Soils: Comparison of Animal Models, Dosing Schemes, and Biological End Points.
机构信息
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, China.
Centre for Environmental Risk Assessment and Remediation, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
出版信息
Environ Sci Technol. 2016 Jan 5;50(1):453-61. doi: 10.1021/acs.est.5b04552. Epub 2015 Dec 9.
Different animals and biomarkers have been used to measure the relative bioavailability of arsenic (As-RBA) in contaminated soils. However, there is a lack of As-RBA comparison based on different animals (i.e., swine and mouse) and biomarkers [area under blood As concentration curve (AUC) after a single gavaged dose vs steady-state As urinary excretion (SSUE) and As accumulation in liver or kidney after multiple doses via diet]. In this study, As-RBA in 12 As-contaminated soils with known As-RBA via swine blood AUC model were measured by mouse blood AUC, SSUE, and liver and kidney analyses. As-RBA ranges for the four mouse assays were 2.8-61%, 3.6-64%, 3.9-74%, and 3.4-61%. Compared to swine blood AUC assay (7.0-81%), though well correlated (R(2) = 0.83), the mouse blood AUC assay yielded lower values (2.8-61%). Similarly, strong correlations of As-RBA were observed between mouse blood AUC and mouse SSUE (R(2) = 0.86) and between urine, liver, and kidney (R(2) = 0.75-0.89), suggesting As-RBA was congruent among different animals and end points. Different animals and biomarkers had little impact on the outcome of in vivo assays to validate in vitro assays. On the basis of its simplicity, mouse liver or kidney assay following repeated doses of soil-amended diet is recommended for future As-RBA studies.
不同的动物和生物标志物已被用于测量污染土壤中砷的相对生物可给性（As-RBA）。然而，基于不同动物（即猪和小鼠）和生物标志物[单次灌胃剂量后血砷浓度曲线下面积（AUC）与稳态砷尿排泄（SSUE）和多次通过饮食给予的砷在肝或肾中的积累]，缺乏 As-RBA 比较。在这项研究中，通过小鼠血 AUC、SSUE 和肝、肾分析，用已知通过猪血 AUC 模型的 As-RBA 的 12 个受砷污染的土壤来测量小鼠血 AUC、SSUE 和肝、肾分析的 As-RBA。四种小鼠检测方法的 As-RBA 范围分别为 2.8-61%、3.6-64%、3.9-74%和 3.4-61%。与猪血 AUC 检测方法（7.0-81%）相比，尽管相关性良好（R²=0.83），但小鼠血 AUC 检测方法的结果较低（2.8-61%）。同样，小鼠血 AUC 与小鼠 SSUE（R²=0.86）和尿、肝、肾之间（R²=0.75-0.89）也观察到 As-RBA 的强相关性，表明不同动物和终点的 As-RBA 是一致的。不同的动物和生物标志物对验证体内检测的体外检测结果的影响不大。基于其简单性，建议在未来的 As-RBA 研究中，对土壤添加饮食进行多次重复剂量后，用小鼠肝或肾检测。
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