石房蛤毒素能力验证测试结果，包括参考物质的特性鉴定和稳定性研究。

Results of a Saxitoxin Proficiency Test Including Characterization of Reference Material and Stability Studies.

作者信息

Harju Kirsi, Rapinoja Marja-Leena, Avondet Marc-André, Arnold Werner, Schär Martin, Luginbühl Werner, Kremp Anke, Suikkanen Sanna, Kankaanpää Harri, Burrell Stephen, Söderström Martin, Vanninen Paula

机构信息

VERIFIN (Finnish Institute for Verification of the Chemical Weapons Convention),Department of Chemistry, P.O. Box 55, A. I. Virtasen aukio 1, University of Helsinki,FI-00014 Helsinki, Finland.

Federal Department of Defence, Civil Protection and Sport, SPIEZ LABORATORY, Austrasse 1,CH-3700 Spiez, Switzerland.

出版信息

Toxins (Basel). 2015 Nov 25;7(12):4852-67. doi: 10.3390/toxins7124852.

DOI:10.3390/toxins7124852

PMID:26602927

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4690102/

Abstract

A saxitoxin (STX) proficiency test (PT) was organized as part of the Establishment of Quality Assurance for the Detection of Biological Toxins of Potential Bioterrorism Risk (EQuATox) project. The aim of this PT was to provide an evaluation of existing methods and the European laboratories' capabilities for the analysis of STX and some of its analogues in real samples. Homogenized mussel material and algal cell materials containing paralytic shellfish poisoning (PSP) toxins were produced as reference sample matrices. The reference material was characterized using various analytical methods. Acidified algal extract samples at two concentration levels were prepared from a bulk culture of PSP toxins producing dinoflagellate Alexandrium ostenfeldii. The homogeneity and stability of the prepared PT samples were studied and found to be fit-for-purpose. Thereafter, eight STX PT samples were sent to ten participating laboratories from eight countries. The PT offered the participating laboratories the possibility to assess their performance regarding the qualitative and quantitative detection of PSP toxins. Various techniques such as official Association of Official Analytical Chemists (AOAC) methods, immunoassays, and liquid chromatography-mass spectrometry were used for sample analyses.

摘要

作为“潜在生物恐怖主义风险生物毒素检测质量保证体系建立”（EQuATox）项目的一部分，组织了一次石房蛤毒素（STX）能力验证测试（PT）。本次能力验证测试的目的是评估现有方法以及欧洲实验室对实际样品中STX及其一些类似物进行分析的能力。制备了含有麻痹性贝类毒素（PSP）的贻贝匀浆材料和藻类细胞材料作为参考样品基质。使用各种分析方法对参考材料进行了表征。从产PSP毒素的甲藻亚历山大藻大量培养物中制备了两种浓度水平的酸化藻类提取物样品。对制备的能力验证测试样品的均匀性和稳定性进行了研究，发现其符合要求。此后，将八个STX能力验证测试样品发送给来自八个国家的十个参与实验室。该能力验证测试为参与实验室提供了评估其在PSP毒素定性和定量检测方面性能的机会。样品分析采用了各种技术，如官方分析化学师协会（AOAC）的官方方法、免疫测定法和液相色谱 - 质谱法。