甲基化DNA免疫沉淀法[MeDIP]用于研究细胞命运决定和癌症发展过程中的表观遗传重塑

The Methylated DNA Immunoprecipitation [MeDIP] to Investigate the Epigenetic Remodeling in Cell Fate Determination and Cancer Development.

作者信息

Masciarelli Silvia, Bellissimo Teresa, Iosue Ilaria, Fazi Francesco

机构信息

Section of Histology & Medical Embryology, Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Sapienza University of Rome, Via A. Scarpa, 14-16, 00161, Rome, Italy.

出版信息

Methods Mol Biol. 2016;1379:69-76. doi: 10.1007/978-1-4939-3191-0_6.

DOI:10.1007/978-1-4939-3191-0_6

PMID:26608290

Abstract

Epigenetic mechanisms such as DNA methylation, posttranslational modifications of histone proteins, remodeling of nucleosomes, and the expression of noncoding RNAs contribute to the regulation of gene expression for the cell fate determination and tissue development. The disruption of these epigenetic mechanisms, in conjunction with genetic alterations, is a decisive element for cancer development and progression. The cancer phenotype is characterized by global DNA hypomethylation and gene-specific hypermethylation. The methylated DNA immunoprecipitation [MeDIP] is a useful approach currently used to clarify the functional consequences of DNA methylation on cell fate determination and cancer development.

摘要

诸如DNA甲基化、组蛋白的翻译后修饰、核小体重塑以及非编码RNA的表达等表观遗传机制，有助于调节基因表达以决定细胞命运和组织发育。这些表观遗传机制的破坏，连同基因改变，是癌症发生和进展的决定性因素。癌症表型的特征是全基因组DNA低甲基化和基因特异性高甲基化。甲基化DNA免疫沉淀法（MeDIP）是目前用于阐明DNA甲基化对细胞命运决定和癌症发展的功能影响的一种有用方法。