敲低真核生物翻译起始因子3D（eIF3D）可通过抑制Wnt/β-连环蛋白信号通路来抑制乳腺癌细胞的增殖和侵袭。
Knockdown of eIF3D inhibits breast cancer cell proliferation and invasion through suppressing the Wnt/β-catenin signaling pathway.
作者信息
Fan Yan, Guo Yufei
机构信息
Department of Galactophore, Henan Province Hospital of Traditional Chinese Medicine Zhengzhou 450002, China.
出版信息
Int J Clin Exp Pathol. 2015 Sep 1;8(9):10420-7. eCollection 2015.
Abstract
eIF3D (eukaryotic translation initiation factor 3 subunit D) is one member of the eIF3 family and plays a critical role in translation initiation. Previous studies showed that it was involved in the development and progression of several tumors. However, the role of eIF3D in breast cancer and the underlying mechanism is still unclear. Therefore, this study set out to investigate the role of eIF3D in breast cancer. Our results demonstrated that eIF3D is up-regulated in breast cancer cells. Knockdown of eIF3D inhibited breast cancer cell proliferation and invasion. In addition, knockdown of eIF3D inhibited the expression of β-catenin, cyclin D1 and c-Myc in breast cancer cells. Taken together, our findings show that siRNA-eIF3D inhibits breast cancer cell proliferation and invasion through suppressing the Wnt/β-catenin signaling pathway. Therefore, eIF3D may be a good molecular target for the prevention and treatment of breast cancer.
摘要
真核生物翻译起始因子3亚基D（eIF3D）是eIF3家族的成员之一，在翻译起始过程中起关键作用。先前的研究表明，它参与了多种肿瘤的发生和发展。然而，eIF3D在乳腺癌中的作用及其潜在机制仍不清楚。因此，本研究旨在探讨eIF3D在乳腺癌中的作用。我们的结果表明，eIF3D在乳腺癌细胞中上调。敲低eIF3D可抑制乳腺癌细胞的增殖和侵袭。此外，敲低eIF3D可抑制乳腺癌细胞中β-连环蛋白、细胞周期蛋白D1和c-Myc的表达。综上所述，我们的研究结果表明，siRNA-eIF3D通过抑制Wnt/β-连环蛋白信号通路来抑制乳腺癌细胞的增殖和侵袭。因此，eIF3D可能是预防和治疗乳腺癌的一个良好分子靶点。
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