Li Shi-Guo, Hou Jing, Liu Xin-Hui, Cui Bao-Shan, Bai Jun-Hong
School of Life Sciences, Tsinghua University, Beijing, China.
State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, China.
Environ Toxicol Chem. 2016 Jul;35(7):1751-8. doi: 10.1002/etc.3315. Epub 2016 Mar 29.
The carcinogenic, teratogenic, and mutagenic effects of hexavalent chromium (Cr[VI]) on living organisms through the food chain raise the immediate need to assess the potential toxicological impacts of Cr(VI) on human health. Therefore, the concentration-dependent responses of 12 Cr(VI)-responsive genes selected from a high-throughput Lycopersicon esculentum complementary DNA microarray were examined at different Cr concentrations. The results indicated that most of the genes were differentially expressed from 0.1 mg Cr/kg soil, whereas the lowest-observable-adverse-effect concentrations of Cr(VI) were 1.6 mg Cr/kg soil, 6.4 mg Cr/kg soil, 3.2 mg Cr/kg soil, and 0.4 mg Cr/kg soil for seed germination, root elongation, root biomass, and root morphology, respectively, implying that the transcriptional method was more sensitive than the traditional method in detecting Cr(VI) toxicity. Dose-dependent responses were observed for the relative expression of expansin (p = 0.778), probable chalcone-flavonone isomerase 3 (p = -0.496), and 12S seed storage protein CRD (p = -0.614); therefore, the authors propose the 3 genes as putative biomarkers in Cr(VI)-contaminated soil. Environ Toxicol Chem 2016;35:1751-1758. © 2015 SETAC.
六价铬（Cr[VI]）通过食物链对生物体产生的致癌、致畸和致突变作用，使得迫切需要评估Cr(VI)对人类健康的潜在毒理学影响。因此，研究人员检测了从高通量番茄互补DNA微阵列中选出的12个Cr(VI)响应基因在不同Cr浓度下的浓度依赖性反应。结果表明，大多数基因在土壤中Cr浓度为0.1mg/kg时就出现差异表达，而对于种子萌发、根伸长、根生物量和根形态而言，Cr(VI)的最低可观察到的有害效应浓度分别为1.6mg Cr/kg土壤、6.4mg Cr/kg土壤、3.2mg Cr/kg土壤和0.4mg Cr/kg土壤，这意味着转录方法在检测Cr(VI)毒性方面比传统方法更敏感。对于扩张蛋白（p = 0.778）、可能的查尔酮-黄酮异构酶3（p = -0.496）和12S种子贮藏蛋白CRD（p = -0.614）的相对表达，观察到了剂量依赖性反应；因此，作者提议将这3个基因作为Cr(VI)污染土壤中的假定生物标志物。《环境毒理学与化学》2016年；35：1751 - 1758。© 2015 SETAC。
