Yan L, Yao Y, Wang L H, Wang M L, Fu X H
Department of Respiratory Diseases, the Affiliated Hospital of the Inner Mongolia Medical University, Hohhot, China.
School of Life Sciences, Inner Mongolia University, Hohhot, China.
Genet Mol Res. 2015 Nov 25;14(4):15090-5. doi: 10.4238/2015.November.24.17.
The expression of CK19, LUNX, and KS1/4 mRNA biomarkers was detected in the peripheral blood of non-small cell lung cancer (NSCLC) patients to investigate the feasibility of indicating lung cancer micrometastases. Micrometastases were identified in the peripheral blood of 32 NSCLC patients, 15 benign pulmonary disease (BPD) patients, and 10 healthy volunteers by reverse transcriptase-polymerase chain reaction. The detection rates of CK19, LUNX, and KS1/4 mRNA-positive cells in the peripheral blood obtained from the NSCLC group were 34.4% (11/32), 37.5% (12/32), and 25% (8/32), respectively. CK19, LUNX, and KS1/4 mRNA-positive cells were detected in 6.6% (1/15), 0.0% (0/15), and 13.3% (2/15) of the patients with BPD, respectively. However, the healthy group did not express any of the three markers. The expression of CK19, LUNX, and KS1/4 mRNA was significantly higher in the NSCLC group than that in the healthy and BPD groups (P < 0.05). CK19 and LUNX mRNA may be ideal biomarkers indicating micrometastases in patients with NSCLC; however, the diagnostic applicability of KS1/4 mRNA remains uncertain. The rate of expression of CK19 was not correlated with the clinicopathological characteristics (P > 0.05). The rate of expression of LUNX and KS1/4 was closely related to the clinical stage (P < 0.05), and not related to the clinical characteristics of the disease (age, gender, smoking history, pathological type, histologic classification, and differentiation; P > 0.05).
检测非小细胞肺癌（NSCLC）患者外周血中CK19、LUNX和KS1/4 mRNA生物标志物的表达，以探讨其用于指示肺癌微转移的可行性。通过逆转录聚合酶链反应在32例NSCLC患者、15例良性肺疾病（BPD）患者和10名健康志愿者的外周血中鉴定微转移。NSCLC组外周血中CK19、LUNX和KS1/4 mRNA阳性细胞的检出率分别为34.4%（11/32）、37.5%（12/32）和25%（8/32）。BPD患者中分别有6.6%（1/15）、0.0%（0/15）和13.3%（2/15）检测到CK19、LUNX和KS1/4 mRNA阳性细胞。然而，健康组未表达这三种标志物中的任何一种。NSCLC组中CK19、LUNX和KS1/4 mRNA的表达显著高于健康组和BPD组（P<0.05）。CK19和LUNX mRNA可能是指示NSCLC患者微转移的理想生物标志物；然而，KS1/4 mRNA的诊断适用性仍不确定。CK19的表达率与临床病理特征无关（P>0.05）。LUNX和KS1/4的表达率与临床分期密切相关（P<0.05），与疾病的临床特征（年龄、性别、吸烟史、病理类型、组织学分类和分化程度；P>0.05）无关。
