Duodu Eugenia, Kraskouskaya Dziyana, Gómez-Biagi Rodolfo F, Gunning Patrick T
Department of Chemistry and Department of Chemical and Physical Sciences, University of Toronto, Mississauga, 3359 Mississauga Road North, Mississauga, Ontario, CanadaL5L1C6.
Analyst. 2016 Feb 7;141(3):820-2. doi: 10.1039/c5an01414j. Epub 2015 Dec 8.
The presence of small phospho-anions, such as PPi and ATP in protein samples often complicates the robust detection of phosphoproteins by metal-based chemosensors and receptors. We herein report the development of a bis(Zn(2+)-cyclen)-triethylbenzene scaffold which can selectively sequester PPi and ATP without affecting the detection of a di-phosphorylated peptide by a ProxyPhos chemosensor.
蛋白质样品中存在的小磷酸阴离子，如焦磷酸（PPi）和三磷酸腺苷（ATP），常常会使基于金属的化学传感器和受体对磷酸化蛋白的可靠检测变得复杂。我们在此报告了一种双（锌（2+）-轮环）-三乙苯支架的开发，该支架可以选择性地螯合焦磷酸和三磷酸腺苷，而不影响ProxyPhos化学传感器对双磷酸化肽的检测。
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