显微切割的黑色素瘤石蜡包埋存档组织基因组DNA的原位亚硫酸氢钠修饰
In Situ Sodium Bisulfite Modification of Genomic DNA from Microdissected Melanoma Paraffin-Embedded Archival Tissues.
作者信息
Marzese Diego M, Huang Sharon K, Hoon Dave S B
机构信息
Department of Molecular Oncology, John Wayne Cancer Institute, Providence Saint John's Health Center, Santa Monica, CA, 90404, USA.
出版信息
Methods Mol Biol. 2015 Dec 13. doi: 10.1007/7651_2015_303.
Abstract
DNA methylation analysis of paraffin-embedded archival tumor tissues (PEAT) is important in clinical and translational research studies. Efficient identification and isolation of homogeneous cell population, optimal DNA extraction, and sodium bisulfite modification (SBM) are essential, particularly in small tumor lesions. Laser capture microdissection (LCM) coupled with an in situ SBM improves the specificity, through histopathology accuracy, and the amount of genomic DNA modified for downstream methylation assays.
摘要
石蜡包埋存档肿瘤组织（PEAT）的DNA甲基化分析在临床和转化研究中具有重要意义。高效识别和分离同质细胞群体、优化DNA提取以及亚硫酸氢钠修饰（SBM）至关重要，尤其是在小肿瘤病变中。激光捕获显微切割（LCM）与原位SBM相结合，通过组织病理学准确性提高了特异性，以及用于下游甲基化检测的基因组DNA修饰量。
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