采用液相色谱-串联质谱法对尿液中的丁丙诺啡、去甲丁丙诺啡、丁丙诺啡葡萄糖醛酸苷、去甲丁丙诺啡葡萄糖醛酸苷和纳洛酮进行定量分析。
Quantitation of Buprenorphine, Norbuprenorphine, Buprenorphine Glucuronide, Norbuprenorphine Glucuronide, and Naloxone in Urine by LC-MS/MS.
作者信息
Marin Stephanie J, McMillin Gwendolyn A
机构信息
ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT, 84108, USA.
ARUP Laboratories, Salt Lake City, UT, USA.
出版信息
Methods Mol Biol. 2016;1383:69-78. doi: 10.1007/978-1-4939-3252-8_8.
Buprenorphine is an opioid drug that has been used to treat opioid dependence on an outpatient basis, and is also prescribed for managing moderate to severe pain. Some formulations of buprenorphine also contain naloxone to discourage misuse. The major metabolite of buprenorphine is norbuprenorphine. Both compounds are pharmacologically active and both are extensively metabolized to their glucuronide conjugates, which are also active metabolites. Direct quantitation of the glucuronide conjugates in conjunction with free buprenorphine, norbuprenorphine, and naloxone in urine can distinguish compliance with prescribed therapy from specimen adulteration intended to mimic compliance with prescribed buprenorphine. This chapter quantitates buprenorphine, norbuprenorphine, their glucuronide conjugates and naloxone directly in urine by liquid chromatography tandem mass spectrometry (LC-MS/MS). Urine is pretreated with formic acid and undergoes solid phase extraction (SPE) prior to analysis by LC-MS/MS.
丁丙诺啡是一种阿片类药物，已被用于门诊治疗阿片类药物依赖，也被用于治疗中度至重度疼痛。丁丙诺啡的一些制剂还含有纳洛酮以防止滥用。丁丙诺啡的主要代谢产物是去甲丁丙诺啡。这两种化合物都具有药理活性，并且都被广泛代谢为它们的葡萄糖醛酸共轭物，这些也是活性代谢产物。尿液中葡萄糖醛酸共轭物与游离丁丙诺啡、去甲丁丙诺啡和纳洛酮的直接定量分析可以区分是否遵守规定治疗，还是通过掺假标本模仿遵守规定的丁丙诺啡治疗。本章通过液相色谱串联质谱法（LC-MS/MS）直接对尿液中的丁丙诺啡、去甲丁丙诺啡、它们的葡萄糖醛酸共轭物和纳洛酮进行定量分析。尿液先用甲酸预处理，然后在通过LC-MS/MS分析之前进行固相萃取（SPE）。
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