血小板中的多巴胺浓度在头颈部副神经节瘤患者中升高。

Dopamine concentration in blood platelets is elevated in patients with head and neck paragangliomas.

出版信息

Clin Chem Lab Med. 2016 Aug 1;54(8):1395-401. doi: 10.1515/cclm-2015-0631.

DOI:10.1515/cclm-2015-0631

Abstract

BACKGROUND

Plasma 3-methoxytyramine (3-MT), a metabolite of dopamine, is elevated in up to 28% of patients with head and neck paragangliomas (HNPGLs). As free dopamine is incorporated in circulating platelets, we determined dopamine concentration in platelets in patients with a HNPGL.

METHODS

A single center cohort study was performed between 2012 and 2014. Thirty-six patients with a HNPGL were compared to healthy controls (68 for dopamine in platelets and 120 for plasma 3-MT).

RESULTS

Dopamine concentration in platelets was elevated in HNPGL patients compared to healthy controls (median [interquartile ranges] 0.48 [0.32-0.82] pmol/109 platelets vs. 0.31 [0.24-0.47] pmol/109 platelets; p<0.05), whereas plasma 3-MT concentration did not differ between both groups (0.06 [0.06-0.08] nmol/L vs. 0.06 [0.06-0.06] nmol/L; p=0.119). Based on 68 healthy controls, the reference interval for dopamine concentration in platelets was 0.12-0.97 pmol/109 platelets. Six (16.7%) patients with a HNPGL demonstrated an increased dopamine concentration in platelets compared to three (8.3%) patients with an increased plasma 3-MT level (p=0.053). The sensitivity and specificity were 16.7% and 98.5% for platelet dopamine and 8.3% and 97.5% for plasma 3-MT concentration (p=0.37).

CONCLUSIONS

Dopamine concentration in platelets is elevated in patients with a HNPGL compared to healthy subjects, and may be a novel biomarker for dopamine producing paraganglioma.

摘要

背景

血浆 3-甲氧基酪胺（3-MT）是多巴胺的一种代谢物，在高达 28%的头颈部副神经节瘤（HNPGL）患者中升高。由于游离多巴胺被纳入循环血小板，因此我们测定了 HNPGL 患者血小板中的多巴胺浓度。

方法

在 2012 年至 2014 年期间进行了一项单中心队列研究。将 36 例 HNPGL 患者与健康对照者（血小板中的多巴胺为 68 例，血浆 3-MT 为 120 例）进行比较。

结果

与健康对照组相比，HNPGL 患者的血小板多巴胺浓度升高（中位数[四分位间距]0.48[0.32-0.82]pmol/109 血小板 vs. 0.31[0.24-0.47]pmol/109 血小板；p<0.05），而两组间血浆 3-MT 浓度无差异（0.06[0.06-0.08]nmol/L vs. 0.06[0.06-0.06]nmol/L；p=0.119）。基于 68 例健康对照者，血小板多巴胺浓度的参考区间为 0.12-0.97 pmol/109 血小板。与 3 例血浆 3-MT 水平升高的患者相比（p=0.053），6 例 HNPGL 患者的血小板多巴胺浓度升高（16.7%）。血小板多巴胺的敏感性和特异性分别为 16.7%和 98.5%，血浆 3-MT 浓度的敏感性和特异性分别为 8.3%和 97.5%（p=0.37）。