马来西亚血红扇头蜱中对立克次体、无形体科和伯氏考克斯体的分子筛查
Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia.
作者信息
Watanabe M, Nakao R, Amin-Babjee S M, Maizatul A M, Youn J H, Qiu Y, Sugimoto C, Watanabe M
机构信息
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Division of Education and Collaboration, Research Center for Zoonosis Control, Hokkaido University, Japan.
出版信息
Trop Biomed. 2015 Jun;32(2):390-8.
A total of 44 Rhipicephalus sanguineus ticks collected from 23 dogs from Malaysia were screened for Rickettsia, Anaplasmataceae and Coxiella burnetii. Coxiella burnetii was detected in 59% (26/44) of ticks however Rickettsia and Anaplasmataceae were not detected in any of the ticks. In order to genotype the strains of C. burnetii, multispacer sequence typing (MST) was carried out using three different spacers. One of the spacers; Cox2 successfully amplified a fragment for which the full length sequence of 397 bp was obtained. The sequenced product revealed only a single nucleotide difference with the Cox2.3 type sequence.
从马来西亚的23只狗身上采集了总共44只血红扇头蜱，对其进行立克次氏体、无形体科和伯氏考克斯氏体检测。在59%（26/44）的蜱中检测到伯氏考克斯氏体，然而在任何蜱中均未检测到立克次氏体和无形体科。为了对伯氏考克斯氏体菌株进行基因分型，使用三个不同的间隔区进行多间隔区序列分型（MST）。其中一个间隔区Cox2成功扩增出一个片段，获得了397 bp的全长序列。测序产物与Cox2.3型序列仅显示出一个单核苷酸差异。
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