人类尿液蛋白质组学：肾脏疾病中的分析技术与临床应用
Human Urine Proteomics: Analytical Techniques and Clinical Applications in Renal Diseases.
作者信息
Kalantari Shiva, Jafari Ameneh, Moradpoor Raheleh, Ghasemi Elmira, Khalkhal Ensieh
机构信息
Chronic Kidney Disease Research Center, Labbafinejad Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Basic Science, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Int J Proteomics. 2015;2015:782798. doi: 10.1155/2015/782798. Epub 2015 Nov 29.
Urine has been in the center of attention among scientists of clinical proteomics in the past decade, because it is valuable source of proteins and peptides with a relative stable composition and easy to collect in large and repeated quantities with a noninvasive procedure. In this review, we discuss technical aspects of urinary proteomics in detail, including sample preparation, proteomic technologies, and their advantage and disadvantages. Several recent experiments are presented which applied urinary proteome for biomarker discovery in renal diseases including diabetic nephropathy, immunoglobulin A (IgA) nephropathy, focal segmental glomerulosclerosis, lupus nephritis, membranous nephropathy, and acute kidney injury. In addition, several available databases in urinary proteomics are also briefly introduced.
在过去十年中，尿液一直是临床蛋白质组学科学家关注的焦点，因为它是蛋白质和肽的宝贵来源，其组成相对稳定，且通过非侵入性程序易于大量且重复地收集。在本综述中，我们详细讨论了尿液蛋白质组学的技术方面，包括样品制备、蛋白质组学技术及其优缺点。还介绍了最近的几项实验，这些实验将尿液蛋白质组用于肾脏疾病生物标志物的发现，包括糖尿病肾病、免疫球蛋白A（IgA）肾病、局灶节段性肾小球硬化、狼疮性肾炎、膜性肾病和急性肾损伤。此外，还简要介绍了尿液蛋白质组学中的几个可用数据库。
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