斑马鱼全胚胎原位杂交及切片分析

Zebrafish Whole-Mount In Situ Hybridization Followed by Sectioning.

作者信息

Doganli Canan, Nyengaard Jens Randel, Lykke-Hartmann Karin

机构信息

Smith Cardiovascular Research Institute, University of California, San Francisco, CA, 94158-9001, USA.

Stereology and Electron Microscopy Laboratory, Centre for Stochastic Geometry and Advanced Bioimaging, Aarhus University Hospital, Aarhus University, Aarhus C, Denmark.

出版信息

Methods Mol Biol. 2016;1377:353-63. doi: 10.1007/978-1-4939-3179-8_31.

DOI:10.1007/978-1-4939-3179-8_31

Abstract

In situ hybridization is a powerful technique used for locating specific nucleic acid targets within morphologically preserved tissues and cell preparations. A labeled RNA or DNA probe hybridizes to its complementary mRNA or DNA sequence within a sample. Here, we describe RNA in situ hybridization protocol for whole-mount zebrafish embryos.

摘要

原位杂交是一种用于在形态学上保存完好的组织和细胞制剂中定位特定核酸靶标的强大技术。标记的RNA或DNA探针与样品中与其互补的mRNA或DNA序列杂交。在此，我们描述了针对整装斑马鱼胚胎的RNA原位杂交方案。

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