切尔诺贝利灾难后遭受电离辐射人群中DNA修复基因XRCC1和XPD多态性与甲状腺癌发生风险的研究

Research of DNA repair genes polymorphism XRCC1 and XPD and the risks of thyroid cancer development in persons exposed to ionizing radiation after the Chornobyl disaster.

作者信息

Shkarupa V M, Henyk-Berezovska S O, Palamarchuk V O, Talko V V, Klymenko S V

机构信息

State Institution National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine, Melnikov str., 53, Kyiv, 04050, Ukraine.

State Institution Institute of Hereditary Pathology National Academy of Medical Sciences of Ukraine, Lviv 79000, Lysenka 31A.

出版信息

Probl Radiac Med Radiobiol. 2015 Dec;20:552-71.

PMID:26695931

Abstract

OBJECTIVE

The objective of this work was to determine and compare the features of DNA repair gene XRCC1 Arg399Gln and XPD Lys751Gln polymorphisms in patients with thyroid cancer (TC), who were exposed to ionizing radiation (IR) as a result of the Chornobyl disaster, and in patients without exposure to ionizing radiation in history.

MATERIALS AND METHODS

Determination of gene XRCC1 Arg399Gln and XPD Lys751Gln polymorphisms was performed by polymerase chain reaction (PCR) in 102 patients with thyroid cancer: 38 people, who were exposed to ionizing radiation due to Chornobyl disaster (members of the accident, and evacuees and residents from controlled areas contaminated with radionuclides), 64 individuals without exposure to ionizing radiation in history and 41 persons residents of Ukraine without cancer pathology in the control group. For comparison of the data on spontaneous and radiation-associated thyroid cancer and settlement of allele frequencies differences and risk of cancer pathology were used the literature data on control groups of populations of Russia, Belarus and Poland.

RESULTS

Comparing to the literature data on XRCC1 Arg399Gln polymorphisms in radiation-exposed individuals without cancer pathology, the risk of thyroid cancer in homozygous minor allele XRCC1 Gln399Gln carriers, who were exposed to ionizing radiation was significantly increased: OR = 4,14, p = 0,001 (CI95 % 1,72-9,93). In homozygous carriers of the minor allele of the gene XPD Lys751Gln, exposed to IR, revealed increased risk of thyroid cancer: OR = 3,30, p = 0,05 (CI 95 % 0,82-14,14), when compared with the control group of Ukrainian population.

CONCLUSIONS

The carriage of homozygous minor allele Gln399Gln XRCC1 and XPD Gln751Gln of DNA repair genes is a risk factor for thyroid cancer under the influence of ionizing radiation in research group of Ukrainian population.

摘要

目的

本研究旨在确定并比较因切尔诺贝利灾难遭受电离辐射（IR）的甲状腺癌（TC）患者与无电离辐射暴露史的甲状腺癌患者中DNA修复基因XRCC1 Arg399Gln和XPD Lys751Gln多态性的特征。

材料与方法

采用聚合酶链反应（PCR）对102例甲状腺癌患者进行基因XRCC1 Arg399Gln和XPD Lys751Gln多态性检测，其中38例因切尔诺贝利灾难遭受电离辐射（事故参与者、撤离者以及来自受放射性核素污染控制区的居民），64例无电离辐射暴露史，对照组为41例无癌症病理的乌克兰居民。为比较自发性和辐射相关性甲状腺癌的数据以及等位基因频率差异和癌症病理风险，使用了俄罗斯、白俄罗斯和波兰人群对照组的文献数据。

结果

与无癌症病理的辐射暴露个体中XRCC1 Arg399Gln多态性的文献数据相比，遭受电离辐射的纯合次要等位基因XRCC1 Gln399Gln携带者患甲状腺癌的风险显著增加：OR = 4.14，p = 0.001（95%CI 1.72 - 9.93）。与乌克兰人群对照组相比，遭受电离辐射的基因XPD Lys751Gln次要等位基因纯合携带者患甲状腺癌的风险增加：OR = 3.30，p = 0.05（95%CI 0.82 - 14.14）。