在适度和高剂量饮食补充条件下，绿猴（埃塞俄比亚绿猴亚种）维生素D的遗传力及妊娠状态的影响。
Vitamin D heritability and effect of pregnancy status in Vervet monkeys (Chlorocebus aethiops sabaeus) under conditions of modest and high dietary supplementation.
作者信息
Chittoor Geetha, Pajewski Nicholas M, Voruganti V Saroja, Comuzzie Anthony G, Clarkson Thomas B, Nudy Matthew, Schnatz Peter F, Kaplan Jay R, Jorgensen Matthew J
机构信息
Department of Nutrition, University of North Carolina at Chapel Hill, Chapel Hill, Kannapolis, NC.
UNC Nutrition Research Institute, University of North Carolina at Chapel Hill, Chapel Hill, Kannapolis, NC.
出版信息
Am J Phys Anthropol. 2016 Apr;159(4):639-45. doi: 10.1002/ajpa.22923. Epub 2015 Dec 28.
OBJECTIVES
The two objectives of the current study were to: 1) investigate the genetic contributions to variations in serum vitamin D concentrations under two dietary conditions (a standard monkey biscuit diet vs. a diet designed to model typical American consumption); and 2) explore the interaction of vitamin D with pregnancy status using a cohort of pedigreed female vervet/African green monkeys.
METHODS
This study includes 185 female (≥3.5 years) vervet/African green monkeys (Chlorocebus aethiops sabaeus) from a multi-generational, pedigreed breeding colony. The 25(OH)D3 concentrations were first measured seven to eight weeks after consuming a "typical American" diet (TAD), deriving 37, 18, and 45% of calories from fat, protein sources, and carbohydrates, and supplemented with vitamin D to a human equivalent of 1,000 IU/day. Vitamin D concentrations were assessed again when animals were switched to a low-fat, standard biscuit diet (LabDiet 5038) for 8 months, which provided a human equivalent of approximately 4,000 IU/day of vitamin D. All statistical analyses were implemented in SOLAR.
RESULTS
Pregnancy was associated with reduced 25(OH)D3 concentrations. Heritability analyses indicated a significant genetic contribution to 25(OH)D3 concentrations in the same monkeys consuming the biscuit diet (h(2) =0.66, P=0.0004) and TAD (h(2) =0.67, P=0.0078) diets, with higher 25(OH)D3 concentrations in animals consuming the biscuit diet. Additionally, there was a significant genotype-by-pregnancy status interaction on 25(OH)D3 concentrations (P<0.05) only among animals consuming the TAD diet.
DISCUSSION
These results support the existence of a genetic contribution to differences in serum 25(OH)D3 concentrations by pregnancy status and emphasize the role of diet (including vitamin D supplementation) in modifying genetic signals as well as vitamin D concentrations.
目的
本研究的两个目的是：1）研究在两种饮食条件下（标准猴饼干饮食与模拟典型美国饮食结构的饮食）血清维生素D浓度变化的遗传贡献；2）使用一群有系谱的雌性绿猴/非洲绿猴来探索维生素D与妊娠状态之间的相互作用。
方法
本研究包括来自一个多代系谱繁殖群体的185只雌性（≥3.5岁）绿猴/非洲绿猴（埃塞俄比亚绿猴亚种）。在食用“典型美国”饮食（TAD）七至八周后首次测量25(OH)D3浓度，该饮食中37%、18%和45%的热量分别来自脂肪、蛋白质来源和碳水化合物，并补充维生素D至相当于人类每日1000国际单位的量。当动物转而食用低脂标准饼干饮食（LabDiet 5038）8个月时再次评估维生素D浓度，该饮食提供相当于人类每日约4000国际单位的维生素D。所有统计分析均在SOLAR中进行。
结果
妊娠与25(OH)D3浓度降低有关。遗传力分析表明，在食用饼干饮食（h(2)=0.66，P=0.0004）和TAD饮食（h(2)=0.67，P=0.0078）的同一批猴子中，25(OH)D3浓度存在显著的遗传贡献，食用饼干饮食的动物25(OH)D3浓度更高。此外，仅在食用TAD饮食的动物中，25(OH)D3浓度存在显著的基因型与妊娠状态的相互作用（P<0.05）。
讨论
这些结果支持血清25(OH)D3浓度差异存在遗传贡献，且强调了饮食（包括维生素D补充）在改变遗传信号以及维生素D浓度方面的作用。
Zotero 插件