Huang Fujiang, Liu Mingdian, Yu Lixiong, Liu Shaoping
a Key Laboratory of Freshwater Fish Reproduction and Development , Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University , Beibei , Chongqing , P.R. China and.
b Yangtze River Fisheries Research Institute of Chinese Academy of Fishery Science, Scientific Observing and Experimental Station of Fishery and Environment in the Upper and Middle Reaches of the Yangtze River, Ministry of Agriculture , Wuhan , Hubei , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Jan;28(1):60-61. doi: 10.3109/19401736.2015.1110798. Epub 2015 Dec 28.
The complete mitogenome sequence of Glyptothorax laosensis was determined using long PCR reactions. The genome is 16 539 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The gene order and composition of G. laosensis was similar to that of most other vertebrates. The descending order of the base composition on heavy strand was 31.3% A, 27.5% C, 25.9% T, 15.3% G, with a relatively lower level of G and a slight AT bias of 57.2%. The results of phylogenetic reconstruction showed G. laosensis is the sister group with G. trilineatus (Figure 1), and the Glyptothorax genus formed a monophyletic group.
利用长PCR反应测定了老挝纹胸鮡的完整线粒体基因组序列。该基因组长度为16539 bp,包括13个典型的脊椎动物蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和一个控制区。除了8个tRNA和ND6基因外,所有其他线粒体基因都位于重链上。老挝纹胸鮡的基因顺序和组成与大多数其他脊椎动物相似。重链上碱基组成的降序排列为A占31.3%、C占27.5%、T占25.9%、G占15.3%,G含量相对较低,AT偏倚率为57.2%。系统发育重建结果表明,老挝纹胸鮡是三线纹胸鮡的姐妹群(图1),纹胸鮡属形成了一个单系类群。