使用十六烷基-4-[¹²⁴I]碘苯甲酸酯进行细胞追踪，评估脂肪来源干细胞在大鼠心肌梗死模型中的安全性和有效性。
Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[¹²⁴I]iodobenzoate for cell tracking.
作者信息
Kim Min Hwan, Lee Kyo Chul, An Gwang Il, Woo Sang-Keun, Park Noh Won, Kim Byung Il, Eom Ki Dong, Kim Kwang Il, Lee Tae Sup, Kim Chan Wha, Yoo Jeongsoo, Kang Joo Hyun, Lee Yong Jin
机构信息
Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea; School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
出版信息
Appl Radiat Isot. 2016 Feb;108:116-123. doi: 10.1016/j.apradiso.2015.12.039. Epub 2015 Dec 17.
This study was aimed to evaluate the effect of (124)I-labeling with hexadecyl-4-iodobenzoate (HIB) on gene expression related to cell cycle, DNA repair, transcription, proliferation and differentiation of adipose-derived stem cells (ADSCs). [(124)I]HIB showed high labeling efficiency with ADSCs (51.3±1.3%, 0.3-2.0 Bq/cell) and there is no morphological change of ADSCs. In the microarray analysis of gene expression pattern, differences were not observed between non-labeled and [(124)I]HIB-labeled ADSCs. We demonstrated that (124)I-labeling with HIB did not affect the biological properties of ADSCs.
本研究旨在评估用十六烷基-4-碘苯甲酸酯（HIB）进行¹²⁴I标记对脂肪来源干细胞（ADSCs）细胞周期、DNA修复、转录、增殖和分化相关基因表达的影响。¹²⁴I-HIB对ADSCs显示出高标记效率（51.3±1.3%，0.3 - 2.0 Bq/细胞），且ADSCs无形态变化。在基因表达模式的微阵列分析中，未标记的ADSCs和¹²⁴I-HIB标记的ADSCs之间未观察到差异。我们证明用HIB进行¹²⁴I标记不影响ADSCs的生物学特性。
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