Department of Nuclear Medicine and Institute of Radiation Medicine, Seoul National University College of Medicine, Seoul, Korea; Department of Radiation Applied Life Science, Seoul National University College of Medicine, Seoul, Korea.
Department of Nuclear Medicine and Institute of Radiation Medicine, Seoul National University College of Medicine, Seoul, Korea.
Nanomedicine. 2016 May;12(4):871-879. doi: 10.1016/j.nano.2015.12.368. Epub 2015 Dec 29.
We tried to develop a dual-modal PET/MR imaging probe using a straightforward one-pot method by encapsulation with specific amphiphiles. In this study, iron oxide (IO) nanoparticles were encapsulated with three amphiphiles containing PEG, DOTA and the prostate-specific membrane antigen (PSMA)-targeting ligand in aqueous medium. The diameter of the prepared nanoparticle DOTA-IO-GUL was 11.01±1.54nm. DOTA-IO-GUL was labeled with (68)Ga in high efficiency. The DOTA-IO-GUL showed a dose-dependent binding to LNCaP (PSMA positive) cells via a competitive binding study against (125)I-labeled MIP-1072 (PSMA-targeting agent). Additionally, PET and MR imaging results showed PSMA selective uptake by only 22Rv1 (PSMA positive) but not PC-3 (PSMA negative) in dual-tumor xenograft mouse model study. MR imaging showed high resolution, and PET imaging enabled quantification and confirmation of the specificity. In conclusion, we have successfully developed the specific PSMA-targeting IO nanoparticle, DOTA-IO-GUL, as a dual-modality probe for complementary PET/MR imaging.
The combination of using Positron Emission Tomography (PET) and computed tomography (CT) in clinical practice is now the norm. With advances in technology, the next step would be to develop combined PET and Magnetic Resonance (MR) dual-imaging. In this article, the authors described their positive study on the development of a dual-modal PET/MR imaging probe using a prostate cancer model.
我们试图通过使用特定的两亲性物质进行封装，通过简单的一锅法开发一种双模态 PET/MR 成像探针。在这项研究中，氧化铁（IO）纳米颗粒被三种包含 PEG、DOTA 和前列腺特异性膜抗原（PSMA）靶向配体的两亲性物质在水介质中包裹。所制备的纳米颗粒 DOTA-IO-GUL 的直径为 11.01±1.54nm。DOTA-IO-GUL 以高效率标记（68）Ga。通过与（125）I 标记的 MIP-1072（PSMA 靶向剂）的竞争性结合研究，DOTA-IO-GUL 表现出与 LNCaP（PSMA 阳性）细胞的剂量依赖性结合。此外，PET 和 MR 成像结果表明，在双肿瘤异种移植小鼠模型研究中，只有 22Rv1（PSMA 阳性）而不是 PC-3（PSMA 阴性）摄取 PSMA 选择性。MR 成像显示高分辨率，PET 成像能够定量和确认特异性。总之，我们已经成功开发了特定的 PSMA 靶向 IO 纳米颗粒 DOTA-IO-GUL，作为互补 PET/MR 成像的双模态探针。
在临床实践中，将正电子发射断层扫描（PET）和计算机断层扫描（CT）结合使用现在已经成为常规。随着技术的进步，下一步将是开发结合 PET 和磁共振（MR）的双成像。在这篇文章中，作者描述了他们在使用前列腺癌模型开发双模态 PET/MR 成像探针方面的积极研究。
