Furuie Hiroki, Yamada Kazuo, Ichitani Yukio
Institute of Psychology and Behavioral Neuroscience, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan; Center for Medical Sciences, Ibaraki Prefectural University of Health Sciences, Ami, Ibaraki 300-0331, Japan.
Institute of Psychology and Behavioral Neuroscience, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.
Behav Brain Res. 2016 Mar 15;301:253-7. doi: 10.1016/j.bbr.2015.12.044. Epub 2015 Dec 31.
Early postnatal glutamatergic N-methyl-d-aspartate (NMDA) receptor blockade in animals is known to produce various behavioral deficits in adulthood. In the present study rats postnatally (day 7-20) treated chronically with MK-801, an NMDA receptor antagonist, were tested later in adulthood in methamphetamine (MAP)-induced conditioned place preference (CPP) using a unbiased procedure in a three-compartment apparatus. Rats with the same chronic treatment in adulthood were also tested. CPP test consisted of a baseline test before conditioning, place conditioning, and a preference test after conditioning. Rats postnatally treated with MK-801 did not show any evidence of preference for MAP-paired compartment compared with that for unpaired one in the preference test that was shown in rats postnatally treated with saline. On the other hand, rats treated with MK-801 in adulthood were not affected by the treatment and showed significant CPP as was shown in saline-treated control animals. Results suggest the possibility that chronic early postnatal, but not adulthood, NMDA receptor blockade induces persistent deficit of subsequent appetitive classical conditioning.
已知在动物出生后早期阻断谷氨酸能N-甲基-D-天冬氨酸（NMDA）受体会在成年期产生各种行为缺陷。在本研究中，出生后（第7 - 20天）长期用NMDA受体拮抗剂MK-801处理的大鼠，成年后在三室装置中使用无偏程序进行甲基苯丙胺（MAP）诱导的条件性位置偏爱（CPP）测试。成年期接受相同慢性处理的大鼠也进行了测试。CPP测试包括条件化前的基线测试、位置条件化以及条件化后的偏爱测试。与出生后用生理盐水处理的大鼠在偏爱测试中表现出对与MAP配对隔室的偏爱相比，出生后用MK-801处理的大鼠没有显示出对MAP配对隔室的任何偏爱证据。另一方面，成年期用MK-801处理的大鼠不受该处理影响，并且如生理盐水处理的对照动物一样表现出显著的CPP。结果表明，出生后早期而非成年期慢性阻断NMDA受体可能会导致随后的食欲经典条件反射持续存在缺陷。
