a Department of Orthopedics , General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA , Shenyang , Liaoning Province , China.
b First Department of Leading-Treating , General Hospital of Shenyang Military Area Command of Chinese PLA , Shenyang , Liaoning Province , China.
Artif Cells Nanomed Biotechnol. 2017 Feb;45(1):108-114. doi: 10.3109/21691401.2015.1129629. Epub 2016 Jan 13.
Mouse bone marrow mesenchymal stem cells C3H10T1/2 were divided into Ad-BMP2 (bone morphogenetic protein 2) group, Ad-VEGF165 (vascular endothelial growth factor 165) group, Ad-VEGF165 + Ad-BMP2 group, empty adenovirus group and control group. BMP2 and VEGF165 were highly co-expressed in Ad-VEGF165 + Ad-BMP2 group. Ad-BMP2 and Ad-VEGF165 + Ad-BMP2 groups, especially the latter (P < 0.05), had significantly higher expression levels of osteocalcin, osteoprotegerin, and osteopontin mRNA and OPN protein (P < 0.05). Ad-VEGF165 + Ad-BMP2 group had highest alkaline phosphatase (ALP) activity, strongest ALP staining and most calcium salt deposits (P < 0.05). Combining VEGF165 obviously enhanced the inducing effects of BMP2 on osteogenic differentiation capacity of C3H10T1/2 cells.
将小鼠骨髓间充质干细胞 C3H10T1/2 分为 Ad-BMP2（骨形态发生蛋白 2）组、Ad-VEGF165（血管内皮生长因子 165）组、Ad-VEGF165+Ad-BMP2 组、空载腺病毒组和对照组。Ad-VEGF165+Ad-BMP2 组中高效共表达 BMP2 和 VEGF165。Ad-BMP2 组和 Ad-VEGF165+Ad-BMP2 组，特别是后者（P<0.05），骨钙素、骨保护素和骨桥蛋白 mRNA 和 OPN 蛋白的表达水平显著升高（P<0.05）。Ad-VEGF165+Ad-BMP2 组碱性磷酸酶（ALP）活性最高，ALP 染色最强，钙盐沉积最多（P<0.05）。联合 VEGF165 明显增强了 BMP2 对 C3H10T1/2 细胞成骨分化能力的诱导作用。
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