Sivan Arun, Shriram A N, Sugunan A P, Anwesh Maile, Muruganandam N, Kartik C, Vijayachari P
Regional Medical Research Centre (Indian Council of Medical Research), Post Bag No. 13, Port Blair, Andaman & Nicobar Islands 744 101, India.
Regional Medical Research Centre (Indian Council of Medical Research), Post Bag No. 13, Port Blair, Andaman & Nicobar Islands 744 101, India.
Acta Trop. 2016 Apr;156:122-9. doi: 10.1016/j.actatropica.2016.01.015. Epub 2016 Jan 15.
From May to June 2014, an outbreak of dengue virus (DENV) illness occurred in the Havelock Island, South Andaman. Entomological investigations were undertaken during the peak of the outbreak, from 26th May-4th June, to identify the primary vector(s) involved in the transmission so that appropriate public health measures could be implemented. Adult mosquitoes were collected by BG-Sentinel traps in houses and neighborhoods of clinically ill patients. Water holding containers were inspected for the presence of mosquito larvae and pupae. Adult mosquitoes were analyzed by RT-PCR for the presence of nucleic acids of DENV and CHIKV. A total of 498 mosquitoes were collected and processed in 27 pools. The species composition comprised of 58.3% Aedes albopictus, 7.5% Aedes aegypti and 4.2% Aedes edwardsi and 3.1% constituted others. Two A. albopictus pools were found to be positive for DENV RNA. Sequencing of the RT PCR 511 base pair amplicon positive samples showed homology with DENV-3, suggesting that serotype-3 was responsible for the outbreak and A. albopictus was the primary vector responsible. This was supported by high container (10.1%), premise (25.4%) and Breteau (27.9) indices, with miscellaneous receptacles (2.4%), tree holes (1.2%) and discarded tires (1.2%) registering relatively higher container indices. This is the first report of detection of DENV in A. albopictus from Andaman and Nicobar Islands.
2014年5月至6月，安达曼南部的哈夫洛克岛发生了登革热病毒（DENV）疫情。在疫情高峰期（5月26日至6月4日）开展了昆虫学调查，以确定传播过程中的主要病媒，从而能够实施适当的公共卫生措施。通过BG-哨兵诱捕器在临床患病患者的房屋和社区收集成年蚊子。检查盛水容器中是否存在蚊虫幼虫和蛹。通过RT-PCR分析成年蚊子，检测是否存在DENV和基孔肯雅病毒（CHIKV）的核酸。共收集了498只蚊子，并分成27组进行处理。种类构成包括58.3%的白纹伊蚊、7.5%的埃及伊蚊、4.2%的爱德华兹伊蚊，其他种类占3.1%。发现两组白纹伊蚊样本的DENV RNA呈阳性。RT-PCR 511碱基对扩增子阳性样本的测序显示与DENV-3具有同源性，表明3型血清型是此次疫情的病原体，白纹伊蚊是主要病媒。高容器指数（10.1%）、房屋指数（25.4%）和布雷图指数（27.9）支持了这一结论，其中杂物容器（2.4%）、树洞（1.2%）和废弃轮胎（1.2%）的容器指数相对较高。这是安达曼和尼科巴群岛首次报道在白纹伊蚊中检测到DENV。
