Nakayama Kosuke, Ohmori Takeshi, Ishikawa Satoshi, Iwata Natsumi, Seto Yasuo, Kawahara Kazuyoshi
a Department of Biosciences, College of Science and Engineering , Kanto Gakuin University , Yokohama , Japan.
b Third Department of Forensic Science , National Research Institute of Police Science , Kashiwa , Japan.
Biosci Biotechnol Biochem. 2016 May;80(5):1024-6. doi: 10.1080/09168451.2015.1123606. Epub 2016 Jan 19.
The plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis was modified to be transferred back to this bacterium. The replication function of S. amiense plasmid was inserted at downstream of OPH gene, and S. fuliginis was transformed with this plasmid. The transformant produced larger amount of active OPH with His-tag than E. coli.
从鞘氨醇单胞菌中克隆得到的编码带有组氨酸标签的有机磷水解酶（OPH）的质粒被改造后再导入该细菌。将氨基鞘氨醇单胞菌质粒的复制功能插入到OPH基因下游，并用此质粒转化鞘氨醇单胞菌。与大肠杆菌相比，该转化体产生了更多带有组氨酸标签的活性OPH。
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