Fielder P J, Robleto D O, Ogren L, Talamantes F
Biology Department, University of California, Santa Cruz 95064.
Am J Physiol. 1989 Oct;257(4 Pt 1):E561-6. doi: 10.1152/ajpendo.1989.257.4.E561.
Hormonal and metabolic responses to ethanol consumption were studied in pregnant Swiss Webster mice and their fetuses. On day 10 of pregnancy, mice were assigned to one of three liquid diet groups: 36% ethanol-derived calories fed ad libitum (ETOH); control liquid diet fed ad libitum (control); control liquid diet administered in the same amount as that consumed by the ETOH group on the same day of pregnancy [pair-fed controls (PF)]. Dams were killed on days 14-18 of pregnancy, and fetuses were collected on days 16-18. Serum mouse placental lactogen-II (mPL-II), prolactin (mPRL), growth hormone (mGH), insulin, and glucose concentrations were determined for each group. Ethanol consumption resulted in a significant reduction in fetal weight on day 18 of pregnancy. The maternal serum mPRL concentration was reduced in ETOH mice on days 16 and 17 of pregnancy when compared with control mice, but when compared with PF mice, it was significantly reduced only on day 16. Maternal and fetal serum insulin concentrations were reduced in ETOH mice when compared with control mice, but it is unclear whether this was a specific effect of ethanol consumption or due to reduced food intake. Maternal serum glucose concentrations were reduced in the ETOH mice but not as severely as in the PF mice. Ethanol consumption had no effect on maternal or fetal serum mPL-II and mGH concentrations. These data confirm that ethanol consumption during pregnancy results in fetal growth retardation, but they do not indicate any major effects of ethanol on mPL-II, mPRL, mGH, and insulin concentrations in either the mother or fetus.
研究了怀孕的瑞士韦伯斯特小鼠及其胎儿对乙醇摄入的激素和代谢反应。在怀孕第10天,将小鼠分为三个液体饮食组之一:自由采食含36%乙醇热量的饮食(ETOH组);自由采食对照液体饮食(对照组);给予与ETOH组在同一天摄入的相同量的对照液体饮食[配对喂养对照组(PF组)]。在怀孕第14 - 18天处死母鼠,并在第16 - 18天收集胎儿。测定每组小鼠血清中的小鼠胎盘催乳素-II(mPL-II)、催乳素(mPRL)、生长激素(mGH)、胰岛素和葡萄糖浓度。乙醇摄入导致怀孕第18天胎儿体重显著降低。与对照小鼠相比,ETOH组母鼠在怀孕第16天和17天血清mPRL浓度降低,但与PF组小鼠相比,仅在第16天显著降低。与对照小鼠相比,ETOH组母鼠和胎儿血清胰岛素浓度降低,但尚不清楚这是乙醇摄入的特异性作用还是由于食物摄入量减少所致。ETOH组母鼠血清葡萄糖浓度降低,但不如PF组严重。乙醇摄入对母鼠或胎儿血清mPL-II和mGH浓度没有影响。这些数据证实,孕期摄入乙醇会导致胎儿生长迟缓,但并未表明乙醇对母鼠或胎儿的mPL-II、mPRL、mGH和胰岛素浓度有任何重大影响。