Desirò Alessandro, Salvioli Alessandra, Bonfante Paola
Department of Life Sciences and Systems Biology, University of Turin, Viale Mattioli 25, Turin, 48824, Italy.
Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, 10125, USA.
Methods Mol Biol. 2016;1399:29-53. doi: 10.1007/978-1-4939-3369-3_2.
The study of the so-called unculturable bacteria is still considered a challenging task. However, given recent improvements in the sensitivity of culture-free approaches, the identification and characterization of such microbes in complex biological samples is now possible. In this chapter we report how endobacteria thriving inside arbuscular mycorrhizal fungi (AMF), which are themselves obligate biotrophs of plants, can be studied using a combination of in vitro culture, molecular biology, and microscopy techniques.
对所谓不可培养细菌的研究仍然被认为是一项具有挑战性的任务。然而，鉴于近年来免培养方法灵敏度的提高，现在有可能对复杂生物样品中的此类微生物进行鉴定和表征。在本章中，我们报告了如何结合体外培养、分子生物学和显微镜技术来研究内生细菌，这些内生细菌在丛枝菌根真菌（AMF）内部生长，而AMF本身是植物的专性生物营养体。
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