Liu Shuai, Li Linge, Tong Changqing, Zhao Qiancheng, Lukyanov Pavel A, Chernikov Oleg V, Li Wei
Alkali Soil Natural Environmental Science Center, Northeast Forestry University/Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, PR China.
College of Life Science, Northeast Forestry University, Harbin 150040, PR China.
Int J Biol Macromol. 2016 Apr;85:530-8. doi: 10.1016/j.ijbiomac.2016.01.034. Epub 2016 Jan 13.
A Ca(2+)-dependent GalNAc/Man-specific lectin (CSL) from Cyclina sinensis was isolated, and its stimulatory action was characterized in yeast. CSL showed a potent effect on the production of ethanol by Saccharomyces cerevisiae. In this work, the changes in the protein expression profiles of S. cerevisiae after 24h of incubation with CSL were analyzed using label-free quantitative proteomics. A total of 1410 proteins were identified, but only 117 proteins showed significant differences in normalized volume (p<0.05). Among the latter proteins, 24 proteins were up-regulated, and 93 were down-regulated. Analysis of the proteome revealed that CSL triggered changes in the concentrations of some enzymes, such as increased expression of hexokinase, glyceraldehyde 3-phosphate dehydrogenase and enolase and decreased expression of dihydrolipoamide dehydrogenase and aldehyde dehydrogenase. These results indicate that CSL can cause some changes in the metabolic pathway involved in ethanol synthesis in S. cerevisiae. These data may help us understand the stimulatory mechanism of lectin in the fermentation process.
从中华圆田螺中分离出一种Ca(2+)依赖的GalNAc/Man特异性凝集素(CSL),并在酵母中对其刺激作用进行了表征。CSL对酿酒酵母产生乙醇具有显著作用。在这项工作中,使用无标记定量蛋白质组学分析了酿酒酵母与CSL孵育24小时后蛋白质表达谱的变化。共鉴定出1410种蛋白质,但只有117种蛋白质在标准化体积上显示出显著差异(p<0.05)。在后者的蛋白质中,24种蛋白质上调,93种蛋白质下调。蛋白质组分析表明,CSL引发了一些酶浓度的变化,例如己糖激酶、甘油醛-3-磷酸脱氢酶和烯醇酶的表达增加,而二氢硫辛酰胺脱氢酶和醛脱氢酶的表达降低。这些结果表明,CSL可导致酿酒酵母中乙醇合成相关代谢途径发生一些变化。这些数据可能有助于我们理解凝集素在发酵过程中的刺激机制。