Zhang Sihuan, Wu Xianfeng, Pan Chuanying, Lei Chuzhao, Dang Ruihua, Chen Hong, Lan Xianyong
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, PR China.
Gene. 2016 Apr 25;581(1):14-20. doi: 10.1016/j.gene.2016.01.013. Epub 2016 Jan 12.
Eukaryotic translation elongation factor 1 delta (EEF1D) gene encodes guanine nucleotide exchange protein eEF1Bδ, which participates in the eukaryotic protein synthesis, and plays important roles in regulating cell cycling and milk production. This study firstly focused on detecting the isoforms of dairy goat EEF1D gene and their mRNA expression characterization. Herein, two novel isoforms, EEF1Da and EEF1Dc, were identified in dairy goat. The entire coding sequences of EEF1Da and EEF1Dc isoforms were 843bp and 267bp in length, respectively. Goat EEF1Da had complete conserved domains of elongation factor 1 (EF1) family, and the evolution of goat EEF1Da isoform was agreed with the evolution of species. Expression pattern analysis of different isoforms revealed relatively ubiquitous expression of EEF1D and EEF1Da. While EEF1Dc only expressed in heart, lung, kidney, adipose and muscle. Combining with the analysis results of cloning, qRT-PCR and bioinformatics, EEF1Da is the major alternative splicing form of EEF1D gene. Interestingly, qRT-PCR result showed that the highest expression of EEF1D was in adipose, which is the major component of mammary. This result was consistent with the early research that EEF1D expressed highly in the mammary, which indicated that EEF1D played a potential key role in regulating adipose development and milk production. All these findings would provide a foundation for the further research of EEF1D gene and development of dairy goat industry.
真核生物翻译延伸因子1δ（EEF1D）基因编码鸟嘌呤核苷酸交换蛋白eEF1Bδ，其参与真核生物蛋白质合成，并在调节细胞周期和产奶量方面发挥重要作用。本研究首先聚焦于检测奶山羊EEF1D基因的异构体及其mRNA表达特征。在此，在奶山羊中鉴定出两种新的异构体，EEF1Da和EEF1Dc。EEF1Da和EEF1Dc异构体的完整编码序列长度分别为843bp和267bp。山羊EEF1Da具有延伸因子1（EF1）家族的完整保守结构域，山羊EEF1Da异构体的进化与物种进化一致。不同异构体的表达模式分析显示EEF1D和EEF1Da表达相对普遍。而EEF1Dc仅在心脏、肺、肾、脂肪和肌肉中表达。结合克隆、qRT-PCR和生物信息学分析结果，EEF1Da是EEF1D基因的主要可变剪接形式。有趣的是，qRT-PCR结果显示EEF1D在脂肪中表达最高，脂肪是乳腺的主要组成部分。这一结果与早期研究中EEF1D在乳腺中高表达一致，表明EEF1D在调节脂肪发育和产奶量方面发挥潜在关键作用。所有这些发现将为进一步研究EEF1D基因和奶山羊产业发展提供基础。
