用飞秒 X 射线吸收光谱法观察肌红蛋白中的血红素堆积。
Observing heme doming in myoglobin with femtosecond X-ray absorption spectroscopy.
机构信息
Department of Physics and Chemistry, University of Palermo , Viale delle Scienze, 90128 Palermo, Italy.
LCLS, SLAC National Accelerator Laboratory , Menlo Park, California 94025, USA.
出版信息
Struct Dyn. 2015 May 29;2(4):041713. doi: 10.1063/1.4921907. eCollection 2015 Jul.
Abstract
We report time-resolved X-ray absorption measurements after photolysis of carbonmonoxy myoglobin performed at the LCLS X-ray free electron laser with nearly 100 fs (FWHM) time resolution. Data at the Fe K-edge reveal that the photoinduced structural changes at the heme occur in two steps, with a faster (∼70 fs) relaxation preceding a slower (∼400 fs) one. We tentatively attribute the first relaxation to a structural rearrangement induced by photolysis involving essentially only the heme chromophore and the second relaxation to a residual Fe motion out of the heme plane that is coupled to the displacement of myoglobin F-helix.
摘要
我们在 LCLS 自由电子激光上进行了一氧化碳肌红蛋白光解的时间分辨 X 射线吸收测量，时间分辨率接近 100fs（半峰全宽）。在铁 K 边的数据表明，在卟啉环处的光诱导结构变化分两步发生，较快的（约 70fs）弛豫先于较慢的（约 400fs）弛豫。我们初步将第一个弛豫归因于光解诱导的结构重排，该重排基本上只涉及卟啉环发色团，第二个弛豫归因于残留在卟啉环外的铁原子的运动，该运动与肌红蛋白 F 螺旋的位移相关。
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