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从鸡肉制品和人类中分离出的金黄色葡萄球菌菌株的发生率、毒力因子、抗菌药物耐药性及基因分型

Occurrence, Virulence Factors, Antimicrobial Resistance, and Genotyping of Staphylococcus aureus Strains Isolated from Chicken Products and Humans.

作者信息

El Bayomi Rasha M, Ahmed Heba A, Awadallah Maysa A I, Mohsen Rasha A, Abd El-Ghafar Abeer E, Abdelrahman Mahmoud A

机构信息

1 Department of Food Control, Faculty of Veterinary Medicine, Zagazig University , Zagazig, Egypt .

2 Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University , Zagazig, Egypt .

出版信息

Vector Borne Zoonotic Dis. 2016 Mar;16(3):157-64. doi: 10.1089/vbz.2015.1891. Epub 2016 Jan 25.

Abstract

Staphylococcus aureus in food is a consequence of inadequate hygienic handling and processing, posing a potential risk to public health. The current study aimed to characterize virulence factors, as well as antimicrobial resistance of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) isolated from retail chicken products and hand swabs from vendors in Egypt. In addition, genetic relatedness of the isolates from chicken and humans was evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using protein A as a target. A total of 110 samples were collected from chicken products (n = 80) and vendors (n = 30). Overall, 30 (37.5%) chicken products samples were positive for S. aureus, whereas hand swabs from meat handlers revealed that 18 (60%) were positive. Ten MRSA strains were characterized by the presence of the mecA gene, comprising seven isolates from chicken and three from humans. Virulence-associated factors were evaluated by PCR, revealing that 31.3% of S. aureus isolates harbored the Panton-Valentine leukocidin (PVL) gene, whereas 10.4% were positive for the sea and sed genes each, and only two isolates were positive for γ-hemolysin-associated gene. Genotyping using spa PCR-RFLP showed identical restriction banding patterns of MRSA isolates of human and chicken meat origin, indicating the genetic relatedness of the isolates. To the best of our knowledge, this is the first study to characterize PVL-positive MRSA from chicken products and to utilize spa-RFLP for evaluating the genetic relatedness between MRSA of human and chicken origin in Egypt.

摘要

食品中的金黄色葡萄球菌是卫生处理和加工不当的结果,对公众健康构成潜在风险。本研究旨在对从埃及零售鸡肉产品和摊贩手部拭子中分离出的金黄色葡萄球菌及耐甲氧西林金黄色葡萄球菌(MRSA)的毒力因子和抗菌药物耐药性进行表征。此外,以蛋白A为靶点,通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)评估鸡源和人源分离株的遗传相关性。共从鸡肉产品(n = 80)和摊贩(n = 30)中采集了110份样本。总体而言,30份(37.5%)鸡肉产品样本金黄色葡萄球菌检测呈阳性,而肉类处理人员的手部拭子显示18份(60%)呈阳性。通过mecA基因的存在鉴定出10株MRSA菌株,其中7株来自鸡肉,3株来自人类。通过PCR评估毒力相关因子,结果显示31.3%的金黄色葡萄球菌分离株携带杀白细胞素(PVL)基因,而sea和sed基因的阳性率分别为10.4%,只有2株分离株γ-溶血素相关基因呈阳性。使用spa PCR-RFLP进行基因分型显示,人源和鸡肉源MRSA分离株的限制性条带模式相同,表明这些分离株具有遗传相关性。据我们所知,这是首次对鸡肉产品中PVL阳性MRSA进行表征,并利用spa-RFLP评估埃及人源和鸡源MRSA之间遗传相关性的研究。

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