Pirog T P, Beregova K A, Savenko I V, Shevchuk T A, Iutynska G O
Mikrobiol Z. 2015 Nov-Dec;77(6):2-10.
To study the effect of Nocardia vaccinii IMV B-7405 surfactants on some bacteria (including pathogens of genera Proteus, Staphylococcus, Enterobacter), yeast of Candida species and fungi (Aspergillus niger R-3, Fusarium culmorum T-7).
The antimi- crobial properties of surfactant were determined in suspension culture by Koch method and also by index of the minimum inhibitory concentration. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2:1).
It is shown that the antimicrobial properties of N. vaccinii IMV B-7405 surfactant depended on the degree of purification (supernatant, solution of surfactant), concentration and exposure. Survival of Escherichia coli IEM-1 and Bacillus subtilis BT-2 (both vegetative cells and spores) after treatment for 1-2 hours with surfactants solution and the supernatant (the surfactant concentration 21 µg/ml) was 3-28%. Minimum inhibitory concentrations of N. vaccinii IMV B-7405 surfactants on studied bacteria, yeast and micromycetes were 11.5-85.0; 11.5-22.5 and 165.0-325.0 µ/ml respectively.
Minimum inhibitory concentrations of N. vaccinii IMV B-7405 surfactants are comparable to those of the known microbial surfactants. The possibility of using the supernatant of culture liquid as an effective antimicrobial agent noticeably simplifies and reduces the cost of the technology of its obtaining.
研究牛痘诺卡氏菌IMV B - 7405表面活性剂对某些细菌（包括变形杆菌属、葡萄球菌属、肠杆菌属的病原体）、念珠菌属酵母及真菌（黑曲霉R - 3、禾谷镰刀菌T - 7）的作用。
采用柯赫氏法在悬浮培养中并通过最低抑菌浓度指标测定表面活性剂的抗菌性能。用氯仿和甲醇（2:1）混合液从培养液上清中提取表面活性剂。
结果表明，牛痘诺卡氏菌IMV B - 7405表面活性剂的抗菌性能取决于纯化程度（上清液、表面活性剂溶液）、浓度和作用时间。用表面活性剂溶液和上清液（表面活性剂浓度21μg/ml）处理1 - 2小时后，大肠杆菌IEM - 1和枯草芽孢杆菌BT - 2（包括营养细胞和孢子）的存活率为3% - 28%。牛痘诺卡氏菌IMV B - 7405表面活性剂对所研究的细菌、酵母和霉菌的最低抑菌浓度分别为11.5 - 85.0；11.5 - 22.5和165.0 - 325.0μg/ml。
牛痘诺卡氏菌IMV B - 7405表面活性剂的最低抑菌浓度与已知微生物表面活性剂相当。使用培养液上清作为有效抗菌剂的可能性显著简化并降低了其获取技术的成本。
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