Huang Yan Li, Gao Zhong Feng, Jia Jing, Luo Hong Qun, Li Nian Bing
Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
J Hazard Mater. 2016 May 5;308:173-8. doi: 10.1016/j.jhazmat.2016.01.048. Epub 2016 Jan 21.
A simple, sensitive and label-free electrochemical sensor is developed for detection of Hg(2+) based on the strong and stable T-Hg(2+)-T mismatches. In the presence of Mg(2+), the parallel G-quadruplex structures could be specifically recognized and precipitated in parallel conformation. Therefore, the guanine nanowire was generated on the electrode surface, triggering the electrochemical H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). In this research, a new method of signal amplification for the quantitative detection of Hg(2+) was described based on the direct growth of guanine nanowire via guanine nanowire. Under optimum conditions, Hg(2+) was detected in the range of 100 pM-100 nM, and the detection limit is 33 pM. Compared to the traditional single G-quadruplex label unit, this electrochemical sensor showed high sensitivity and selectivity for detecting Hg(2+).
基于强而稳定的T-Hg(2+)-T错配，开发了一种用于检测Hg(2+)的简单、灵敏且无标记的电化学传感器。在Mg(2+)存在的情况下，平行的G-四链体结构能够被特异性识别并以平行构象沉淀。因此，在电极表面生成了鸟嘌呤纳米线，引发了电化学H2O2介导的3,3',5,5'-四甲基联苯胺（TMB）氧化。在本研究中，描述了一种基于通过鸟嘌呤纳米线直接生长鸟嘌呤纳米线进行Hg(2+)定量检测的信号放大新方法。在最佳条件下，Hg(2+)的检测范围为100 pM至100 nM，检测限为33 pM。与传统的单个G-四链体标记单元相比，这种电化学传感器在检测Hg(2+)时表现出高灵敏度和选择性。
