Pi Jiang, Huang Lufen, Yang Fen, Jiang Jinhuan, Jin Hua, Liu Jianxin, Su Xiaohui, Wu Anguo, Cai Huaihong, Yang Peihui, Cai Jiye
State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.
Department of Pharmacology, Hunan University of Medicine, Huaihua, China.
Scanning. 2016 Nov;38(6):525-534. doi: 10.1002/sca.21291. Epub 2016 Feb 3.
Mast cell degranulation is the typical anaphylaxis process of mast cells associated with the release of cytokines, eicosanoids and their secretory granules, which play very important roles in the allergic inflammatory response of the human body upon anaphylactogen stimulation. The calcium ionophore ionomycin is widely used as a degranulation induction agent for mast cell degranulation studies. In the present work, ionomycin-induced degranulation of RBL-2H3 basophilic leukemia cell line cells was investigated in vitro by high resolution atomic force microscopy (AFM). Ionomycin, which could increase the intracellular free Ca level and β-Hexosaminidase release, was found to induce the formation of a kind of peculiar vesicles in the cytoplasm area of RBL-2H3 cells. Those vesicles induced by ionomycin would desintegrate to release a larger amount of granules surrounding RBL-2H3 cells by the controlling of F-actin. These results provide the precise morphological information of ionomycin-induced mast cell degranulation at nanoscale, which could benefit our understanding of ionomycin-induced mast cell anaphylaxis model and also validate the applicability of AFM for the detection of allergic inflammatory response in mast cells. SCANNING 38:525-534, 2016. © 2016 Wiley Periodicals, Inc.
肥大细胞脱颗粒是肥大细胞典型的过敏反应过程，与细胞因子、类花生酸及其分泌颗粒的释放有关，这些物质在变应原刺激下人体的过敏性炎症反应中发挥着非常重要的作用。钙离子载体离子霉素被广泛用作肥大细胞脱颗粒研究的脱颗粒诱导剂。在本研究中，通过高分辨率原子力显微镜（AFM）在体外研究了离子霉素诱导的RBL-2H3嗜碱性白血病细胞系细胞的脱颗粒情况。发现离子霉素可增加细胞内游离钙水平并释放β-己糖胺酶，从而诱导RBL-2H3细胞胞质区域形成一种特殊的囊泡。离子霉素诱导形成的这些囊泡会在F-肌动蛋白的控制下解体，释放出大量围绕RBL-2H3细胞的颗粒。这些结果提供了纳米级离子霉素诱导肥大细胞脱颗粒的精确形态学信息，这有助于我们理解离子霉素诱导的肥大细胞过敏反应模型，也验证了AFM在检测肥大细胞过敏性炎症反应中的适用性。《扫描》38:525 - 534，2016年。© 2016威利期刊公司。
