Oehmichen M, Schmidt V, Stuka K
Beitr Gerichtl Med. 1989;47:7-11.
Investigation of vitality was done in paraffin-embedded tissue sections of open skin wounds by demonstration of a neutrophilic reaction marking the cells by naphthol AS-D chloroacetate esterase, by demonstration of enzyme activity by application of lysozyme antibodies and by demonstration of concentration of proteinase inhibitors by application of inhibitor antibodies using the indirect immunohistochemical peroxidase method. Survival time of the wounds could be determined in 10 cases (15-165 mins) and was unknown in 9 other cases of sudden death due to injury of the major vessels or heart. Postmortally inflicted injuries and cases of sudden death due to massive blunt trauma served as controls. In vital injuries, accumulation of proteinase inhibitors, particularly alpha-1-antichymotrypsin and alpha-2-macroglobulin, were demonstrable in the corium parallel to the wound surface. Protein accumulation was observed only sporadically in cases of sudden death and never in cases with postmortally inflicted wounds. Granulocytes and lysozyme were first observed in the corium after a survival time of more than 60 minutes.
通过用萘酚AS-D氯乙酸酯酶标记细胞来显示嗜中性反应、应用溶菌酶抗体来显示酶活性以及应用抑制剂抗体通过间接免疫组织化学过氧化物酶法来显示蛋白酶抑制剂的浓度，对开放性皮肤伤口的石蜡包埋组织切片进行活力研究。10例伤口的存活时间可以确定（15 - 165分钟），另外9例因大血管或心脏损伤导致猝死的病例存活时间未知。死后造成的损伤以及因大量钝器伤导致猝死的病例作为对照。在生前损伤中，在真皮中与伤口表面平行处可显示蛋白酶抑制剂的积累，特别是α-1-抗糜蛋白酶和α-2-巨球蛋白。在猝死病例中仅偶尔观察到蛋白质积累，而在死后造成伤口的病例中从未观察到。在存活时间超过60分钟后，首先在真皮中观察到粒细胞和溶菌酶。
