Michel F, Umesono K, Ozeki H
Centre de Génétique Moléculaire du C.N.R.S., 91190 Gif-sur-Yvette, France.
Gene. 1989 Oct 15;82(1):5-30. doi: 10.1016/0378-1119(89)90026-7.
The 70 published sequences of group II introns from fungal and plant mitochondria and plant chloroplasts are analyzed for conservation of primary sequence, secondary structure and three-dimensional base pairings. Emphasis is put on structural elements with known or suspected functional significance with respect to self-splicing: the exon-binding and intron-binding sites, the bulging A residue involved in lariat formation, structural domain V and two isolated base pairs, one of them involving the last intron nucleotide and the other one, the first nt of the 3' exon. Separate sections are devoted to the 29 group II-like introns from Euglena chloroplasts and to the possible relationship of catalytic group II introns to nuclear premessenger introns. Alignments of all available sequences of group II introns are provided in the APPENDIX.
对已发表的来自真菌、植物线粒体及植物叶绿体的70个II组内含子序列进行分析，以研究其一级序列、二级结构及三维碱基配对的保守性。重点关注那些对于自我剪接具有已知或疑似功能意义的结构元件：外显子结合位点和内含子结合位点、参与套索形成的凸起A残基、结构域V以及两个孤立的碱基对，其中一个涉及内含子的最后一个核苷酸，另一个涉及3'外显子的第一个核苷酸。单独的章节专门论述了来自眼虫叶绿体的29个类II组内含子，以及催化性II组内含子与核前体信使内含子之间可能存在的关系。II组内含子所有可用序列的比对情况见附录。
