辣椒（茄科）野生种和栽培种中45S和5S rRNA基因的荧光原位杂交及银染核仁组织区定位

FISH and AgNor mapping of the 45S and 5S rRNA genes in wild and cultivated species of Capsicum (Solananceae).

作者信息

Scaldaferro Marisel A, da Cruz M Victoria Romero, Cecchini Nicolás M, Moscone Eduardo A

机构信息

a Instituto Multidisciplinario de Biología Vegetal (IMBIV), CONICET and Universidad Nacional de Córdoba, CC 495, CP 5000, Córdoba, Argentina.

b Facultad de Ciencias Exactas, Físicas y Naturales (FCEFyN), Universidad Nacional de Córdoba, Av. Vélez Sarsfield 299, CP 5000, Córdoba, Argentina.

出版信息

Genome. 2016 Feb;59(2):95-113. doi: 10.1139/gen-2015-0099.

DOI:10.1139/gen-2015-0099

PMID:26853884

Abstract

Chromosome number and position of rDNA were studied in 12 wild and cultivated species of the genus Capsicum with chromosome numbers x = 12 and x = 13 (22 samples). For the first time in these species, the 5S and 45S rRNA loci were localized and physically mapped using two-color fluorescence in situ hybridization and AgNOR banding. We focused on the comparison of the results obtained with both methods with the aim of accurately revealing the real functional rRNA genes. The analyzes were based on a previous work that reported that the 18S-5.8S-25S loci mostly coincide with GC-rich heterochromatic regions and likely have given rise to satellite DNAs, which are not active genes. These data show the variability of rDNA within karyotypes of the genus Capsicum, providing anchor points for (comparative) genetic maps. In addition, the obtained information might be useful for studies on evolution of repetitive DNA.

摘要

对12种野生和栽培的辣椒属物种（染色体数x = 12和x = 13，共22个样本）的染色体数目和rDNA位置进行了研究。首次在这些物种中，使用双色荧光原位杂交和银染核仁组织区（AgNOR）显带技术对5S和45S rRNA基因座进行了定位和物理图谱绘制。我们着重比较两种方法所得结果，旨在准确揭示真正具有功能的rRNA基因。这些分析基于之前的一项研究，该研究报告称18S - 5.8S - 25S基因座大多与富含GC的异染色质区域重合，并且可能产生了卫星DNA，而这些并非活性基因。这些数据显示了辣椒属核型内rDNA的变异性，为（比较）遗传图谱提供了锚定标记。此外，所获得的信息可能对重复DNA的进化研究有用。