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Electrostatic engineering of the interface between heavy and light chains promotes antibody Fab fragment production.

作者信息

Ohmuro-Matsuyama Yuki, Mori Keita, Hamada Hirotsugu, Ueda Hiroshi, Yamaji Hideki

机构信息

Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.

Manufacturing Technology Association of Biologics (MAB), c/o Center for Business Incubation, Kobe University, 1-5-6 Minatojima-Minamimachi, Chuo-ku, Kobe, 650-0047, Japan.

出版信息

Cytotechnology. 2017 Jun;69(3):469-475. doi: 10.1007/s10616-016-9955-4. Epub 2016 Feb 8.

Abstract

Monoclonal antibodies and antibody fragments are used for diverse diagnostic and therapeutic applications. We have investigated the secretory production of Fab fragments from insect cells cotransfected with plasmid vectors carrying heavy- and light-chain genes. In the present study, to promote the formation of the disulfide bond between the heavy and light chains, some positively charged amino acid residues were introduced near the cysteine residue for the disulfide bond at the C-terminus of C, while some negatively charged amino acid residues were added near the cysteine residue for the disulfide bond at the C-terminus of C. This electrostatic steering led to an increase in Fab secretions from insect cells.

摘要

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