COMP-Ang1通过抑制TLR4信号通路来抑制炎症，从而促进生物惰性多孔腔中同种异体胰岛移植的长期存活。
COMP-Ang1 promotes long-term survival of allogeneic islet grafts in a bioinert perforated chamber by inhibiting inflammation via inhibition of the TLR4 signaling pathway.
作者信息
Wang Dalei, Jiang Lina, Liang Yingzi, Hao Xiaoyan, Chen Chen, Xia Wei, Zhuang Ran, Su Yingjun, Guo Shuzhong
机构信息
Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi'an, 710032, People's Republic of China.
Department of Pathology and Hepatology, Beijing 302 Hospital, Beijing, 100039, People's Republic of China.
出版信息
Biotechnol Lett. 2016 Jun;38(6):1033-42. doi: 10.1007/s10529-016-2059-6. Epub 2016 Feb 13.
OBJECTIVES
To evaluate the effects of cartilage oligomeric matrix protein (COMP)- angiopoietin-1 (Ang1) on allogeneic islet graft survival in a bioinert perforated chamber.
RESULTS
COMP-Ang1 treatment significantly decreased lipopolysaccharide-induced cell apoptosis and islet-related lymph node cell proliferation (both P < 0.01). Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 levels in the chamber exudate were significantly lower in the COMP-Ang1 + chamber group than in the chamber group (all P < 0.05), as were the protein expression levels. COMP-Ang1 significantly inhibited the expression of Toll-like receptor 4 (TLR4) in cultured islets. Finally, full COMP-Ang1 treatment resulted in the longest survival time among the treatment groups.
CONCLUSION
Combined use of the bioinert perforated chamber with COMP-Ang1 is an effective strategy for improving islet allograft survival.
目的
评估软骨寡聚基质蛋白（COMP）-血管生成素-1（Ang1）对生物惰性多孔腔室中同种异体胰岛移植存活的影响。
结果
COMP-Ang1治疗显著降低了脂多糖诱导的细胞凋亡和胰岛相关淋巴结细胞增殖（均P<0.01）。COMP-Ang1+腔室组腔室渗出液中的肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和IL-6水平显著低于腔室组（均P<0.05），蛋白质表达水平也是如此。COMP-Ang1显著抑制培养胰岛中Toll样受体4（TLR4）的表达。最后，全剂量COMP-Ang1治疗导致治疗组中存活时间最长。
结论
生物惰性多孔腔室与COMP-Ang1联合使用是提高胰岛同种异体移植存活的有效策略。
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