一种基于三个基因的风险评分可预测切除的非小细胞肺癌的预后。

A three gene-based risk score predicts prognosis of resected non-small-cell lung cancer.

作者信息

Liu Kun, Chen Hong-Lin, Gu Ming-Ming, You Qing-Sheng

机构信息

Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University Nantong, Jiangsu Province, P. R. China.

Nantong University Nantong, Jiangsu Province, P. R. China.

出版信息

Int J Clin Exp Pathol. 2015 Dec 1;8(12):16081-8. eCollection 2015.

PMID:26884885

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4730098/

Abstract

BACKGROUND

To study the prognosis-predicting value of a risk score based on phosphorylated At (p-Akt), vascular endothelial growth factor (VEGF), and Nin one binding (NOB1) expression in patients with resected non-small-cell lung cancer (NSCLC).

METHODS

A prospective cohort among 98 consecutive patients with resected NSCLC was conducted in 2009 to 2010. Immunohistochemistry was used in the detection of p-Akt, VEGF, and NOB1 expression. Any of three genes with positive expression was allocated a score of 1, otherwise scored 0. The risk score ranged from 0-3. Prognosis outcomes included overall survival (OS) and progression-free survival (PFS). Log-rank test and Cox hazard model were used to investigate the prognosis predicting value for the risk score.

RESULTS

In the 98 NSCLC tissue specimens, p-Akt, VEGF and NOB1 positive Expression rates were 42.9%, 66.3%, and 60.2%, respectively. The median for OS was 44 month, with 95% CI 35-51 months, and the median for PFS was 36 months, with 95% CI 25-49 months. Log-rank test showed OS and PFS related with TMN stage, lymph node metastasis, p-Akt expression, VEGF expression, NOB1 expression, and gene-based risk score (P<0.05). Multivariate COX analysis showed pTMN stage, lymph node metastasis, p-Akt expression, VEGF expression, and gene-based risk score were independent prognosis factors for OS and PFS. The adjusted HR for gene-based risk score with every one score increase was 1.21 [1.04-1.56] for OS and 1.19 [1.02-1.79] for PFS.

CONCLUSIONS

Our results suggest the risk scores based on p-Akt, VEGF, NOB1 expression can predict postoperative survival in patients with resected NSCLC.

摘要

背景

研究基于磷酸化Akt（p-Akt）、血管内皮生长因子（VEGF）和Nin一结合蛋白（NOB1）表达的风险评分对非小细胞肺癌（NSCLC）切除术后患者的预后预测价值。

方法

2009年至2010年对98例连续的NSCLC切除术后患者进行前瞻性队列研究。采用免疫组织化学法检测p-Akt、VEGF和NOB1的表达。三个基因中任何一个呈阳性表达得1分，否则得0分。风险评分范围为0至3分。预后结局包括总生存期（OS）和无进展生存期（PFS）。采用对数秩检验和Cox风险模型研究风险评分的预后预测价值。

结果

在98例NSCLC组织标本中，p-Akt、VEGF和NOB1的阳性表达率分别为42.9%、66.3%和60.2%。OS的中位数为44个月，95%CI为35至51个月；PFS的中位数为36个月，95%CI为25至49个月。对数秩检验显示，OS和PFS与TMN分期、淋巴结转移、p-Akt表达、VEGF表达、NOB1表达及基于基因的风险评分相关（P<0.05）。多因素COX分析显示，pTMN分期、淋巴结转移、p-Akt表达、VEGF表达及基于基因的风险评分是OS和PFS的独立预后因素。基于基因的风险评分每增加1分，OS的调整后HR为1.21[1.04-1.56]，PFS的调整后HR为1.19[1.02-1.79]。