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法国帕万淡水半混合湖缺氧区活性小型微生物真核生物的多样性与动态

Diversity and Dynamics of Active Small Microbial Eukaryotes in the Anoxic Zone of a Freshwater Meromictic Lake (Pavin, France).

作者信息

Lepère Cécile, Domaizon Isabelle, Hugoni Mylène, Vellet Agnès, Debroas Didier

机构信息

Laboratoire "Microorganismes: Génome et Environnement", Clermont Université, Université Blaise PascalClermont-Ferrand, France; Centre National de la Recherche Scientifique, UMR 6023, LMGEAubière, France.

Institut National de la Recherche Agronomique, UMR 42 CARRTELThonon-les-Bains, France; Université Savoie MontBlancChambéry, France.

出版信息

Front Microbiol. 2016 Feb 10;7:130. doi: 10.3389/fmicb.2016.00130. eCollection 2016.

DOI:10.3389/fmicb.2016.00130
PMID:26904006
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4748746/
Abstract

Microbial eukaryotes play a crucial role in ecosystem functioning and oxygen is considered to be one of the strongest barriers against their local dispersal. However, diversity of microbial eukaryotes in freshwater habitats with oxygen gradients has previously received very little attention. We applied high-throughput sequencing (V4 region of the 18S rRNA gene) in conjunction with quantitative PCR (DNA and RNA) and fluorescent in situ hybridization (FISH) analyses, to provide an unique spatio-temporal analysis of microbial eukaryotes diversity and potential activity in a meromictic freshwater lake (lake Pavin). This study revealed a high genetic diversity of unicellular eukaryotes in the permanent anoxic zone of lake Pavin and allowed the discrimination of active vs. inactive components. Forty-two percent of the OTUs (Operational Taxonomic Units) are exclusively present in the monimolimnion, where Alveolata (Ciliophora and Dinophyceae) and Fungi (Dikarya and Chytrids) are the most active phyla and are probably represented by species capable of anaerobic metabolism. Pigmented eukaryotes (Haptophyceae and Chlorophyceae) are also present and active in this zone, which opens up questions regarding their metabolism.

摘要

微生物真核生物在生态系统功能中发挥着关键作用，而氧气被认为是阻碍其在局部扩散的最强屏障之一。然而，此前氧气梯度淡水生境中微生物真核生物的多样性很少受到关注。我们应用高通量测序（18S rRNA基因的V4区域）结合定量PCR（DNA和RNA）以及荧光原位杂交（FISH）分析，对一个半咸水淡水湖（帕万湖）中微生物真核生物的多样性和潜在活性进行独特的时空分析。这项研究揭示了帕万湖永久缺氧区单细胞真核生物的高度遗传多样性，并能够区分活跃成分和非活跃成分。42%的操作分类单元（OTUs）仅存在于均温层，其中囊泡虫类（纤毛虫纲和甲藻纲）和真菌（双核亚界和壶菌门）是最活跃的门类，可能由能够进行厌氧代谢的物种代表。有色素的真核生物（定鞭藻纲和绿藻纲）也存在于该区域并具有活性，这引发了关于其代谢的问题。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/1c2c9c4bc641/fmicb-07-00130-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/9e60af623356/fmicb-07-00130-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/c6c87f0b5854/fmicb-07-00130-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/41ca2fdb3dd2/fmicb-07-00130-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/f04316ffa312/fmicb-07-00130-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/1c2c9c4bc641/fmicb-07-00130-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/9e60af623356/fmicb-07-00130-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/c6c87f0b5854/fmicb-07-00130-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/41ca2fdb3dd2/fmicb-07-00130-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/f04316ffa312/fmicb-07-00130-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8f3/4748746/1c2c9c4bc641/fmicb-07-00130-g0005.jpg

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