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基于转录组学鉴定水稻白叶枯病菌在不同水稻品种中表现出不同毒力的差异表达基因

Transcriptome-Based Identification of Differently Expressed Genes from Xanthomonas oryzae pv. oryzae Strains Exhibiting Different Virulence in Rice Varieties.

作者信息

Noh Tae-Hwan, Song Eun-Sung, Kim Hong-Il, Kang Mi-Hyung, Park Young-Jin

机构信息

National Institute of Crop Science, Rural Development Administration, Wanju-gun 55365, Korea.

National Institute of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea.

出版信息

Int J Mol Sci. 2016 Feb 19;17(2):259. doi: 10.3390/ijms17020259.

DOI:10.3390/ijms17020259
PMID:26907259
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4783988/
Abstract

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight (BB) in rice (Oryza sativa L.). In this study, we investigated the genome-wide transcription patterns of two Xoo strains (KACC10331 and HB1009), which showed different virulence patterns against eight rice cultivars, including IRBB21 (carrying Xa21). In total, 743 genes showed a significant change (p-value < 0.001 in t-tests) in their mRNA expression levels in the HB1009 (K3a race) strain compared with the Xoo KACC10331 strain (K1 race). Among them, four remarkably enriched GO terms, DNA binding, transposition, cellular nitrogen compound metabolic process, and cellular macromolecule metabolic process, were identified in the upregulated genes. In addition, the expression of 44 genes was considerably higher (log2 fold changes > 2) in the HB1009 (K3a race) strain than in the Xoo KACC10331 (K1 race) strain. Furthermore, 13 and 12 genes involved in hypersensitive response and pathogenicity (hrp) and two-component regulatory systems (TCSs), respectively, were upregulated in the HB1009 (K3a race) strain compared with the Xoo KACC10331 (K1 race) strain, which we determined using either quantitative real-time PCR analysis or next-generation RNA sequencing. These results will be helpful to improve our understanding of Xoo and to gain a better insight into the Xoo-rice interactions.

摘要

水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae，简称Xoo）可引发水稻（Oryza sativa L.）白叶枯病。在本研究中，我们调查了两株Xoo菌株（KACC10331和HB1009）的全基因组转录模式，这两株菌株对包括携带Xa21的IRBB21在内的8个水稻品种表现出不同的致病模式。与Xoo KACC10331菌株（K1小种）相比，HB1009（K3a小种）菌株共有743个基因的mRNA表达水平发生了显著变化（t检验中p值<0.001）。其中，在上调基因中鉴定出四个显著富集的GO术语，即DNA结合、转座、细胞氮化合物代谢过程和细胞大分子代谢过程。此外，44个基因在HB1009（K3a小种）菌株中的表达明显高于Xoo KACC10331（K1小种）菌株（log2倍数变化>2）。此外，与Xoo KACC10331（K1小种）菌株相比，HB1009（K3a小种）菌株中分别有13个和12个参与过敏反应和致病性（hrp）以及双组分调节系统（TCSs）的基因上调，这是我们通过定量实时PCR分析或新一代RNA测序确定的。这些结果将有助于增进我们对Xoo的了解，并更好地洞察Xoo与水稻之间的相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55b9/4783988/3bcad8e7c0e9/ijms-17-00259-g005.jpg
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1
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Mol Plant. 2015 Feb;8(2):290-302. doi: 10.1016/j.molp.2014.10.010. Epub 2014 Dec 15.
2
PCR-based assay for rapid and specific detection of the new Xanthomonas oryzae pv. oryzae K3a race using an AFLP-derived marker.基于聚合酶链式反应（PCR）的检测方法，用于使用AFLP衍生标记物快速、特异性地检测新型水稻白叶枯病菌K3a小种。
J Microbiol Biotechnol. 2014 Jun 28;24(6):732-9. doi: 10.4014/jmb.1311.11005.
3
The rice TAL effector-dependent resistance protein XA10 triggers cell death and calcium depletion in the endoplasmic reticulum.
对水稻叶枯病病原菌稻黄单胞菌的全基因组代谢重建和计算机分析。
Mol Plant Pathol. 2020 Apr;21(4):527-540. doi: 10.1111/mpp.12914. Epub 2020 Feb 18.
水稻 TAL 效应因子依赖的抗性蛋白 XA10 在内质网中引发细胞死亡和钙离子耗竭。
Plant Cell. 2014 Jan;26(1):497-515. doi: 10.1105/tpc.113.119255. Epub 2014 Jan 31.
4
The Xanthomonas Ax21 protein is processed by the general secretory system and is secreted in association with outer membrane vesicles.黄单胞菌 Ax21 蛋白通过一般分泌系统加工，并与外膜囊泡一起分泌。
PeerJ. 2014 Jan 7;2:e242. doi: 10.7717/peerj.242. eCollection 2014.
5
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6
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