编码汞还原酶的氧化亚铁硫杆菌染色体基因的核苷酸序列。

Nucleotide sequence of the Thiobacillus ferrooxidans chromosomal gene encoding mercuric reductase.

作者信息

Inoue C, Sugawara K, Shiratori T, Kusano T, Kitagawa Y

机构信息

Laboratory of Plant Genetic Engineering, Akita Prefectural College of Agriculture, Japan.

出版信息

Gene. 1989 Dec 7;84(1):47-54. doi: 10.1016/0378-1119(89)90138-8.

DOI:10.1016/0378-1119(89)90138-8

PMID:2691338

Abstract

The nucleotide sequence of the Thiobacillus ferrooxidans chromosomal mercuric-reductase-encoding gene (merA) has been determined. The merA gene contains 1635 bp, and shares 78.2% and 76.6% sequence homology with the transposon, Tn501, and plasmid R100 merA genes, respectively. From the sequence, a 545-amino acid (aa) polypeptide was deduced, and comparison with those of Tn501 and R100 revealed 80.6% and 80.0% homology, respectively, at the aa sequence level. Divergence among the three merA aa sequences was clustered within a specific region (aa positions 41-87). By analysis of codon usage frequency, it is speculated that the T. ferrooxidans merA gene originated from Tn501, R100, or a common ancestral gene, but not from T. ferrooxidans itself.

摘要

已经测定了氧化亚铁硫杆菌染色体汞还原酶编码基因（merA）的核苷酸序列。merA基因包含1635个碱基对，与转座子Tn501和质粒R100的merA基因的序列同源性分别为78.2%和76.6%。从该序列推导得出一个545个氨基酸的多肽，与Tn501和R100的多肽在氨基酸序列水平上的同源性分别为80.6%和80.0%。这三个merA氨基酸序列之间的差异集中在一个特定区域（氨基酸位置41 - 87）。通过对密码子使用频率的分析，推测氧化亚铁硫杆菌的merA基因起源于Tn501、R100或一个共同的祖先基因，而不是来自氧化亚铁硫杆菌自身。