促性腺激素释放激素相关肽在培养的人垂体细胞中的催乳素抑制活性。

Prolactin-inhibiting activity of GnRH associated peptide in cultured human pituitary cells.

作者信息

Wormald P J, Abrahamson M J, Seeburg P H, Nikolics K, Millar R P

机构信息

Department of Chemical Pathology, University of Cape Town Medical School, Observatory, Republic of South Africa.

出版信息

Clin Endocrinol (Oxf). 1989 Feb;30(2):149-55. doi: 10.1111/j.1365-2265.1989.tb03736.x.

DOI:10.1111/j.1365-2265.1989.tb03736.x

PMID:2692878

Abstract

The 56-amino-acid extension of GnRH in the human GnRH precursor (pHGnRH 14-69 or GAP) has previously been shown to inhibit PRL secretion from cultured rat pituitary cells. We have studied the effect of GAP and shorter sequences on prolactin secretion from human and rat pituitary cells. Bacterially synthesized GAP inhibited PRL secretion from human pituitary cells. At 10(-6) M GAP inhibition of prolactin release was 67.7% which was similar to that observed in rat pituitary cells (65.5%). A series of shorter peptide sequences (pHGnRH 14-26, pHGnRH 14-36, pHGnRH 14-37.NH2, pHGnRH 28-36, pHGnRH 38-49 and pHGnRH 51-66) which are potentially processed from GAP at basic amino acid residues had no effect on prolactin secretion from human or rat pituitary cells at doses up to 10(-5) M.

摘要

人促性腺激素释放激素前体（pHGnRH 14 - 69或GAP）中促性腺激素释放激素的56个氨基酸延伸片段先前已被证明可抑制培养的大鼠垂体细胞分泌催乳素。我们研究了GAP和较短序列对人及大鼠垂体细胞催乳素分泌的影响。细菌合成的GAP抑制了人垂体细胞分泌催乳素。在10⁻⁶ M时，GAP对催乳素释放的抑制率为67.7%，这与在大鼠垂体细胞中观察到的抑制率（65.5%）相似。一系列可能在碱性氨基酸残基处从GAP加工而来的较短肽序列（pHGnRH 14 - 26、pHGnRH 14 - 36、pHGnRH 14 - 37.NH₂、pHGnRH 28 - 36、pHGnRH 38 - 49和pHGnRH 51 - 66）在剂量高达10⁻⁵ M时对人或大鼠垂体细胞的催乳素分泌没有影响。