Rajalaxmi Murugan, Beema Shafreen Rajamohamed, Iyer Prasanth M, Sahaya Vino Raja, Balamurugan Krishnaswamy, Pandian Shunmugiah Karutha
a Department of Biotechnology Science Campus , Alagappa University , Karaikudi , India.
b Center for Nanoscience and Nanotechnology , Sathyabama University , Chennai , India.
Biofouling. 2016;32(4):1-12. doi: 10.1080/08927014.2016.1154545.
Biofilm formation is a major contributing factor in the pathogenesis of Vibrio cholerae O1 (VCO1) and therefore preventing biofilm formation could be an effective alternative strategy for controlling cholera. The present study was designed to explore seawater bacteria as a source of anti-biofilm agents against VCO1. Indole-3-carboxaldehyde (I3C) was identified as an active principle component in Marinomonas sp., which efficiently inhibited biofilm formation by VCO1 without any selection pressure. Furthermore, I3C applications also resulted in considerable collapsing of preformed pellicles. Real-time PCR studies revealed the down-regulation of virulence gene expression by modulation of the quorum-sensing pathway and enhancement of protease production, which was further confirmed by phenotypic assays. Furthermore, I3C increased the survival rate of Caenorhabditis elegans when infected with VCO1 by significantly reducing in vivo biofilm formation, which was corroborated by a survivability assay. Thus, this study revealed, for the first time, the potential of I3C as an anti-biofilm agent against VCO1.
生物膜形成是霍乱弧菌O1型（VCO1）发病机制中的一个主要促成因素，因此防止生物膜形成可能是控制霍乱的一种有效替代策略。本研究旨在探索海水细菌作为抗VCO1生物膜剂的来源。吲哚-3-甲醛（I3C）被鉴定为海单胞菌属中的一种活性主要成分，它能有效抑制VCO1形成生物膜，且无任何选择压力。此外，应用I3C还导致预先形成的菌膜大量瓦解。实时PCR研究表明，通过调节群体感应途径和增强蛋白酶产生，毒力基因表达下调，这一点通过表型分析得到进一步证实。此外，I3C通过显著减少体内生物膜形成，提高了感染VCO1的秀丽隐杆线虫的存活率，生存能力测定也证实了这一点。因此，本研究首次揭示了I3C作为抗VCO1生物膜剂的潜力。
