使用荧光蛋白和彩色成像技术可视化具有不同遗传特性的癌细胞。

Use of fluorescent proteins and color-coded imaging to visualize cancer cells with different genetic properties.

机构信息

AntiCancer Inc., 7917 Ostrow Street, San Diego, CA, 92111, USA.

Department of Surgery, University of California San Diego, San Diego, CA, USA.

出版信息

Cancer Metastasis Rev. 2016 Mar;35(1):5-19. doi: 10.1007/s10555-016-9610-8.

DOI:10.1007/s10555-016-9610-8

PMID:26942457

Abstract

Fluorescent proteins are very bright and available in spectrally-distinct colors, enable the imaging of color-coded cancer cells growing in vivo and therefore the distinction of cancer cells with different genetic properties. Non-invasive and intravital imaging of cancer cells with fluorescent proteins allows the visualization of distinct genetic variants of cancer cells down to the cellular level in vivo. Cancer cells with increased or decreased ability to metastasize can be distinguished in vivo. Gene exchange in vivo which enables low metastatic cancer cells to convert to high metastatic can be color-coded imaged in vivo. Cancer stem-like and non-stem cells can be distinguished in vivo by color-coded imaging. These properties also demonstrate the vast superiority of imaging cancer cells in vivo with fluorescent proteins over photon counting of luciferase-labeled cancer cells.

摘要

荧光蛋白非常亮，并且有光谱上不同的颜色可供选择，能够对体内生长的彩色编码癌细胞进行成像，从而区分具有不同遗传特性的癌细胞。使用荧光蛋白对癌细胞进行非侵入性和活体成像，可以在体内可视化到不同遗传变异的癌细胞，甚至可以达到细胞水平。可以在体内区分具有不同转移能力的癌细胞。可以对体内使低转移癌细胞向高转移转化的基因交换进行彩色成像。可以通过彩色成像在体内区分癌症干细胞样细胞和非干细胞样细胞。这些特性也表明，使用荧光蛋白对体内癌细胞进行成像的优势远远超过对荧光素酶标记的癌细胞进行光子计数。