生长素对轮藻离子运输的影响。

Auxin effects on ion transport in Chara corallina.

作者信息

Zhang Suyun, de Boer Albertus H, van Duijn Bert

机构信息

Plant Biodynamics Laboratory, Institute Biology Leiden, Leiden University, Sylvius Laboratory, Sylviusweg 72, 2333 BE Leiden, The Netherlands.

Department of Structural Biology, Faculty Earth and Life Sciences, Vrije Universiteit, De Boelelaan 1085-1087, 1081HV Amsterdam, The Netherlands.

出版信息

J Plant Physiol. 2016 Apr 1;193:37-44. doi: 10.1016/j.jplph.2016.02.009. Epub 2016 Feb 24.

DOI:10.1016/j.jplph.2016.02.009

PMID:26943501

Abstract

The plant hormone auxin has been widely studied with regard to synthesis, transport, signaling and functions among the land plants while there is still a lack of knowledge about the possible role for auxin regulation mechanisms in algae with "plant-like" structures. Here we use the alga Chara corallina as a model to study aspects of auxin signaling. In this respect we measured auxin on membrane potential changes and different ion fluxes (K(+), H(+)) through the plasma membrane. Results showed that auxin, mainly IAA, could hyperpolarize the membrane potential of C. corallina internodal cells. Ion flux measurements showed that the auxin-induced membrane potential change may be based on the change of K(+) permeability and/or channel activity rather than through the activation of proton pumps as known in land plants.

摘要

植物激素生长素在陆地植物的合成、运输、信号传导和功能方面已得到广泛研究，然而对于具有“类植物”结构的藻类中生长素调节机制的可能作用仍缺乏了解。在此，我们以珊瑚轮藻为模型来研究生长素信号传导的各个方面。在这方面，我们测量了生长素对膜电位变化以及通过质膜的不同离子通量（K⁺、H⁺）的影响。结果表明，生长素（主要是吲哚 - 3 - 乙酸，IAA）可使珊瑚轮藻节间细胞的膜电位超极化。离子通量测量结果显示，生长素诱导的膜电位变化可能基于K⁺通透性和/或通道活性的改变，而不是像陆地植物中那样通过质子泵的激活。