Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Food Chem. 2016 Jul 15;203:386-393. doi: 10.1016/j.foodchem.2016.02.093. Epub 2016 Feb 15.
Green extraction using air-assisted, low-density solvent-based liquid-liquid microextraction and solidified floating organic droplets (AA-LDS-LLME-SFOD) prior to spectrophotometry was successfully applied for quantitation of carotenoids in fruit juices. Under optimal conditions, β-carotene could be quantified with a linear response up to a concentration of 60 μg mL(-1). The procedure was performed in a microcentrifuge tube with 40 μL of 1-dodecanol as the extraction solvent and a 1.0 mL juice sample containing 8% NaCl under seven extraction cycles of air pumping by syringe. This method was validated based on linearity (0.2-30 μg mL(-1), R(2) 0.998), limit of detection (0.04 μg mL(-1)) and limit of quantification (0.13 μg mL(-1)). The precision, expressed as the relative standard deviation (RSD) of the calibration curve slope (n=12), for inter-day and intra-day analysis was 4.85% and 7.92%, respectively. Recovery of β-carotene was in the range of 93.6-101.5%. The newly proposed method is simple, rapid and environmentally friendly, particularly as a useful screening test for food analysis.
采用空气辅助、低密度溶剂基液-液微萃取和固化浮质有机液滴（AA-LDS-LLME-SFOD）进行绿色提取，然后进行分光光度法，成功应用于果汁中类胡萝卜素的定量分析。在最佳条件下，β-胡萝卜素的线性响应可达到 60 μg mL(-1) 的浓度。该程序在微离心管中进行，使用 40 μL 的 1-十二醇作为萃取溶剂，在 7 个注射器抽吸空气的萃取循环中，使用 1.0 mL 含 8%NaCl 的果汁样品。该方法基于线性（0.2-30 μg mL(-1)，R(2) 0.998）、检测限（0.04 μg mL(-1)) 和定量限（0.13 μg mL(-1))进行了验证。日内和日间分析的校准曲线斜率的相对标准偏差（RSD）分别表示为 4.85%和 7.92%。β-胡萝卜素的回收率在 93.6-101.5%之间。该新方法简单、快速、环保，特别适用于食品分析的筛选测试。
