SPIDIA-DNA泛欧外部质量评估的数据与性能评估：第二份SPIDIA-DNA实验室报告
Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report.
作者信息
Malentacchi Francesca, Pizzamiglio Sara, Ibrahim-Gawel Hady, Pazzagli Mario, Verderio Paolo, Ciniselli Chiara Maura, Wyrich Ralf, Gelmini Stefania
机构信息
Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Italy.
Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
出版信息
Data Brief. 2016 Feb 6;6:980-4. doi: 10.1016/j.dib.2016.01.062. eCollection 2016 Mar.
Within the EU-SPIDIA project (www.spidia.eu), the quality parameters of blood genomic DNA were defined [SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses - [1]; Influence of pre-analytical procedures on genomic DNA integrity in blood samples: the SPIDIA experience - [2]; Combining qualitative and quantitative imaging evaluation for the assessment of genomic DNA integrity: the SPIDIA experience - [3]. DNA quality parameters were used to evaluate the laboratory performance within an External Quality Assessment (EQA) [Second SPIDIA-DNA External Quality Assessment (EQA): Influence of pre-analytical phase of blood samples on genomic DNA quality - [4]. These parameters included DNA purity and yield by UV spectrophotometric measurements, the presence of PCR interferences by Kineret software and genomic DNA integrity analysis by Pulsed Field Gel Electrophoresis. Here we present the specific laboratory report of the 2nd SPIDIA-DNA EQA as an example of data and performances evaluation.
在欧盟-SPIDIA项目（www.spidia.eu）中，已定义了血液基因组DNA的质量参数[SPIDIA-DNA：用于基于DNA分析的血液样本分析前阶段的外部质量评估 - [1]；分析前程序对血液样本中基因组DNA完整性的影响：SPIDIA经验 - [2]；结合定性和定量成像评估以评估基因组DNA完整性：SPIDIA经验 - [3]。DNA质量参数用于在外部质量评估（EQA）中评估实验室性能[第二次SPIDIA-DNA外部质量评估（EQA）：血液样本分析前阶段对基因组DNA质量的影响 - [4]。这些参数包括通过紫外分光光度法测量的DNA纯度和产量、Kineret软件检测的PCR干扰以及脉冲场凝胶电泳进行的基因组DNA完整性分析。在此，我们以第二次SPIDIA-DNA EQA的具体实验室报告为例进行数据和性能评估。
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