Avetisyan L R, Chernukha M Yu, Shaginyan I A, Kapranov N I, Siyanova E A, Medvedeva O S, Kondratieva E I, Alekseeva G V, Krasovsky S A, Usacheva M V, Amelina E L
Zh Mikrobiol Epidemiol Immunobiol. 2015 Nov-Dec(6):3-10.
Study the spectrum of resistance to antibiotics and its variability of Staphylococcus aureus, Pseudomonas aeruginosa and Burkholderia cepacia complex (BCC), persisting in lungs of MV patients.
312 strains of S. aureus, 213 strains of P. aeruginosa, 186 strains of BCC were studied. Monitoring of antibiotics sensitivity was carried out in strains, isolated from 30 patients with chronic S. aureus infection, from 22 patients with chronic BCC infection and from 21 patients with chronic pseudomonas infection. Interval of monitoring was from 14 days to 5 years 7 months.
Study of S. aureus, P. aeruginosa and BCC strains has shown, that 35 and 33.3% of cases of staphylococcus infection, 37 and 46% of pseudomonas infection in children and adults, respectively, 100% of BCC infections were determined by multi-resistant clones. Study of genotypically identical strains, isolated from a single patient at different stages, has shown a change in antibiotics sensitivity as a result of persistence.
Persisent infection of lungs in patients with MV is determined: by exchanging clones with varying antibiotics sensitivity or prolonged circulation of a single clone with a high degree of phenotypical and genotypical variability, that determine alteration of seeding of sensitive and resistant strains from the same patient during monitoring. This confirms the necessity of study of antibiotics sensitivity of strains for prescription of antibacterial therapy.
研究耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌和洋葱伯克霍尔德菌复合体（BCC）在机械通气患者肺部持续存在的抗生素耐药谱及其变异性。
研究了312株金黄色葡萄球菌、213株铜绿假单胞菌和186株BCC。对从30例慢性金黄色葡萄球菌感染患者、22例慢性BCC感染患者和21例慢性铜绿假单胞菌感染患者中分离出的菌株进行抗生素敏感性监测。监测间隔为14天至5年7个月。
对金黄色葡萄球菌、铜绿假单胞菌和BCC菌株的研究表明，葡萄球菌感染病例中分别有35%和33.3%、儿童和成人铜绿假单胞菌感染病例中分别有37%和46%、100%的BCC感染由多重耐药克隆引起。对从同一患者不同阶段分离出的基因型相同的菌株进行研究表明，由于持续存在，抗生素敏感性发生了变化。
机械通气患者肺部的持续感染是由以下因素决定的：通过交换具有不同抗生素敏感性的克隆，或单一克隆的长期循环，其具有高度的表型和基因型变异性，这决定了在监测期间同一患者敏感菌株和耐药菌株的接种变化。这证实了研究菌株抗生素敏感性以指导抗菌治疗处方的必要性。
